Effects of intervertebral disc lesion and multifidus muscle resection on the structure of the lumbar intervertebral discs and paraspinal musculature of the rat

Huub Maas*, Wendy Noort, Paul W. Hodges, Jaap van Dieën

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

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Abstract

The aim of this study was to investigate whether elimination of multifidus muscle in rats causes intervertebral disc (IVD) degeneration similar to that found after IVD lesion. Data were obtained from 36 male Wistar rats randomly assigned to one of three groups: (i) IVD lesion, in which the L4/L5 IVD was stabbed; (ii) multifidus muscle resection, in which all multifidus tissue between L3 and L6 was excised bilaterally; (iii) control, in which no intervention was applied. At 7, 14, and 28 days post-intervention, L4/L5 IVDs were harvested for histological analysis; left and right multifidus fascicles between L3 and S1 (from control and IVD lesion animals) and medial longissimus between L1 and S3 (from all animals) were dissected and weighed. ANOVA indicated significant group differences and a significant interaction between group and days for relative nucleus pulposus area and for multifidus mass normalized to body mass. No significant effects were observed for whole IVD area. At 14 days post-op, the IVD lesion group had a significantly smaller relative nucleus pulposus area than control and multifidus resection groups. Nucleus pulposus size did not differ from control at 7 and 28 days. At 7 days post-intervention, normalized multifidus mass was significantly lower (20%) in the IVD lesion group. For longissimus mass, no between-group differences were found. These results indicate that, in rats, IVD recovers quickly after lumbar IVD lesion and multifidus disruption does not cause IVD degeneration within the time studied.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalJournal of Biomechanics
Volume70
Early online date12 Jan 2018
DOIs
Publication statusPublished - 21 Mar 2018

Funding

We would like to thank Magda Kwanten and Guus Baan for help with the surgical procedures and data collection and analysis. PH is supported by a Fellowship from the National Health and Medical Research Council (NHMRC) of Australia ( APP1102905 ). We would like to thank Magda Kwanten and Guus Baan for help with the surgical procedures and data collection and analysis. PH is supported by a Fellowship from the National Health and Medical Research Council (NHMRC) of Australia (APP1102905).

FundersFunder number
Magda Kwanten
National Health and Medical Research CouncilAPP1102905

    Keywords

    • Animal model
    • Intervertebral disc degeneration
    • Low back pain
    • Spinal stability
    • Spine

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