Abstract
The aim of this study was to investigate whether elimination of multifidus muscle in rats causes intervertebral disc (IVD) degeneration similar to that found after IVD lesion. Data were obtained from 36 male Wistar rats randomly assigned to one of three groups: (i) IVD lesion, in which the L4/L5 IVD was stabbed; (ii) multifidus muscle resection, in which all multifidus tissue between L3 and L6 was excised bilaterally; (iii) control, in which no intervention was applied. At 7, 14, and 28 days post-intervention, L4/L5 IVDs were harvested for histological analysis; left and right multifidus fascicles between L3 and S1 (from control and IVD lesion animals) and medial longissimus between L1 and S3 (from all animals) were dissected and weighed. ANOVA indicated significant group differences and a significant interaction between group and days for relative nucleus pulposus area and for multifidus mass normalized to body mass. No significant effects were observed for whole IVD area. At 14 days post-op, the IVD lesion group had a significantly smaller relative nucleus pulposus area than control and multifidus resection groups. Nucleus pulposus size did not differ from control at 7 and 28 days. At 7 days post-intervention, normalized multifidus mass was significantly lower (20%) in the IVD lesion group. For longissimus mass, no between-group differences were found. These results indicate that, in rats, IVD recovers quickly after lumbar IVD lesion and multifidus disruption does not cause IVD degeneration within the time studied.
| Original language | English |
|---|---|
| Pages (from-to) | 228-234 |
| Journal | Journal of Biomechanics |
| Volume | 70 |
| DOIs | |
| Publication status | Published - 21 Mar 2018 |
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Keywords
- Animal model
- Intervertebral disc degeneration
- Low back pain
- Spinal stability
- Spine
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Effects of intervertebral disc lesion and multifidus muscle resection on the structure of the lumbar intervertebral discs and paraspinal musculature of the rat. / Maas, Huub; Noort, Wendy; Hodges, Paul W.; van Dieën, Jaap.
In: Journal of Biomechanics, Vol. 70, 21.03.2018, p. 228-234.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Effects of intervertebral disc lesion and multifidus muscle resection on the structure of the lumbar intervertebral discs and paraspinal musculature of the rat
AU - Maas, Huub
AU - Noort, Wendy
AU - Hodges, Paul W.
AU - van Dieën, Jaap
PY - 2018/3/21
Y1 - 2018/3/21
N2 - The aim of this study was to investigate whether elimination of multifidus muscle in rats causes intervertebral disc (IVD) degeneration similar to that found after IVD lesion. Data were obtained from 36 male Wistar rats randomly assigned to one of three groups: (i) IVD lesion, in which the L4/L5 IVD was stabbed; (ii) multifidus muscle resection, in which all multifidus tissue between L3 and L6 was excised bilaterally; (iii) control, in which no intervention was applied. At 7, 14, and 28 days post-intervention, L4/L5 IVDs were harvested for histological analysis; left and right multifidus fascicles between L3 and S1 (from control and IVD lesion animals) and medial longissimus between L1 and S3 (from all animals) were dissected and weighed. ANOVA indicated significant group differences and a significant interaction between group and days for relative nucleus pulposus area and for multifidus mass normalized to body mass. No significant effects were observed for whole IVD area. At 14 days post-op, the IVD lesion group had a significantly smaller relative nucleus pulposus area than control and multifidus resection groups. Nucleus pulposus size did not differ from control at 7 and 28 days. At 7 days post-intervention, normalized multifidus mass was significantly lower (20%) in the IVD lesion group. For longissimus mass, no between-group differences were found. These results indicate that, in rats, IVD recovers quickly after lumbar IVD lesion and multifidus disruption does not cause IVD degeneration within the time studied.
AB - The aim of this study was to investigate whether elimination of multifidus muscle in rats causes intervertebral disc (IVD) degeneration similar to that found after IVD lesion. Data were obtained from 36 male Wistar rats randomly assigned to one of three groups: (i) IVD lesion, in which the L4/L5 IVD was stabbed; (ii) multifidus muscle resection, in which all multifidus tissue between L3 and L6 was excised bilaterally; (iii) control, in which no intervention was applied. At 7, 14, and 28 days post-intervention, L4/L5 IVDs were harvested for histological analysis; left and right multifidus fascicles between L3 and S1 (from control and IVD lesion animals) and medial longissimus between L1 and S3 (from all animals) were dissected and weighed. ANOVA indicated significant group differences and a significant interaction between group and days for relative nucleus pulposus area and for multifidus mass normalized to body mass. No significant effects were observed for whole IVD area. At 14 days post-op, the IVD lesion group had a significantly smaller relative nucleus pulposus area than control and multifidus resection groups. Nucleus pulposus size did not differ from control at 7 and 28 days. At 7 days post-intervention, normalized multifidus mass was significantly lower (20%) in the IVD lesion group. For longissimus mass, no between-group differences were found. These results indicate that, in rats, IVD recovers quickly after lumbar IVD lesion and multifidus disruption does not cause IVD degeneration within the time studied.
KW - Animal model
KW - Intervertebral disc degeneration
KW - Low back pain
KW - Spinal stability
KW - Spine
UR - http://www.scopus.com/inward/record.url?scp=85040932845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040932845&partnerID=8YFLogxK
U2 - 10.1016/j.jbiomech.2018.01.004
DO - 10.1016/j.jbiomech.2018.01.004
M3 - Article
VL - 70
SP - 228
EP - 234
JO - Journal of Biomechanics
JF - Journal of Biomechanics
SN - 0021-9290
ER -