Effects of ZnO Nanoparticles on Phaseolus vulgaris Germination and Seedling Development Determined by X-ray Spectroscopy

S.M. Savassa, N.M. Duran, E.S. Rodrigues, E. de Almeida, C.A.M. van Gestel, Thiago F.V. Bompadre, Hudson W.P. Carvalho

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

There is still little information on the potential use of nanomaterials for seed nutrient enhancement through seed priming. The use of nanoparticles (NPs) in agriculture is promising, but in-depth knowledge on their interaction with plants is required. The aim of this study was to evaluate the effects of different concentrations (1–5000 mg L–1) and sizes (20, 40, and 60 nm) of uncoated ZnO NP compared to ionic ZnSO4 (positive control) on common bean (Phaseolus vulgaris) seed germination. The seeds were soaked in ZnO NP aqueous dispersions for 20 min. The ZnO nanoparticles did not affect the germination rate. The 10 mg L–1 40 nm ZnO treatment showed a tendency to increase weight after 5 days (8.26 ± 0.11 g) when compared to the negative control (7.7 ± 0.7 g). However, at 5000 mg L–1 40 nm ZnO NP and ZnSO4 weight was reduced to 7.7 ± 0.8 g and 6.05 ± 0.08 g, respectively. Microprobe X-ray fluorescence showed that most of the Zn absorbed was trapped in the seed coat, while a small fraction entered the cotyledon. X-ray absorption spectroscopy indicated the biotransformation of the ZnO NP. In the hilum and cotyledon, Zn was found associated with organic molecules such as citrate, malate, and histidine-like compounds. Seedling weight reduction depended on the concentration of Zn taken up by the tissue and on the biotransformation of ZnO into organically bound Zn. Considering the properties of the studied NP, in particular the slow Zn release and lower toxicity compared to ZnSO4, the results represent a step forward toward the application of ZnO NP as an agrochemical.
Original languageEnglish
Pages (from-to)6414–6426
JournalACS Applied Nano Materials
Volume1
Issue number11
DOIs
Publication statusPublished - 1 Nov 2018

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nanoparticles
Phaseolus vulgaris
spectroscopy
X-radiation
germination
seedlings
biotransformation
cotyledons
seed priming
nanomaterials
agrochemicals
malates
seeds
histidine
citrates
seed germination
beans
weight loss
fluorescence
toxicity

Cite this

Savassa, S.M. ; Duran, N.M. ; Rodrigues, E.S. ; de Almeida, E. ; van Gestel, C.A.M. ; Bompadre, Thiago F.V. ; Carvalho, Hudson W.P. / Effects of ZnO Nanoparticles on Phaseolus vulgaris Germination and Seedling Development Determined by X-ray Spectroscopy. In: ACS Applied Nano Materials. 2018 ; Vol. 1, No. 11. pp. 6414–6426.
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abstract = "There is still little information on the potential use of nanomaterials for seed nutrient enhancement through seed priming. The use of nanoparticles (NPs) in agriculture is promising, but in-depth knowledge on their interaction with plants is required. The aim of this study was to evaluate the effects of different concentrations (1–5000 mg L–1) and sizes (20, 40, and 60 nm) of uncoated ZnO NP compared to ionic ZnSO4 (positive control) on common bean (Phaseolus vulgaris) seed germination. The seeds were soaked in ZnO NP aqueous dispersions for 20 min. The ZnO nanoparticles did not affect the germination rate. The 10 mg L–1 40 nm ZnO treatment showed a tendency to increase weight after 5 days (8.26 ± 0.11 g) when compared to the negative control (7.7 ± 0.7 g). However, at 5000 mg L–1 40 nm ZnO NP and ZnSO4 weight was reduced to 7.7 ± 0.8 g and 6.05 ± 0.08 g, respectively. Microprobe X-ray fluorescence showed that most of the Zn absorbed was trapped in the seed coat, while a small fraction entered the cotyledon. X-ray absorption spectroscopy indicated the biotransformation of the ZnO NP. In the hilum and cotyledon, Zn was found associated with organic molecules such as citrate, malate, and histidine-like compounds. Seedling weight reduction depended on the concentration of Zn taken up by the tissue and on the biotransformation of ZnO into organically bound Zn. Considering the properties of the studied NP, in particular the slow Zn release and lower toxicity compared to ZnSO4, the results represent a step forward toward the application of ZnO NP as an agrochemical.",
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Effects of ZnO Nanoparticles on Phaseolus vulgaris Germination and Seedling Development Determined by X-ray Spectroscopy. / Savassa, S.M.; Duran, N.M.; Rodrigues, E.S.; de Almeida, E.; van Gestel, C.A.M.; Bompadre, Thiago F.V.; Carvalho, Hudson W.P.

In: ACS Applied Nano Materials, Vol. 1, No. 11, 01.11.2018, p. 6414–6426.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - Effects of ZnO Nanoparticles on Phaseolus vulgaris Germination and Seedling Development Determined by X-ray Spectroscopy

AU - Savassa, S.M.

AU - Duran, N.M.

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AU - de Almeida, E.

AU - van Gestel, C.A.M.

AU - Bompadre, Thiago F.V.

AU - Carvalho, Hudson W.P.

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N2 - There is still little information on the potential use of nanomaterials for seed nutrient enhancement through seed priming. The use of nanoparticles (NPs) in agriculture is promising, but in-depth knowledge on their interaction with plants is required. The aim of this study was to evaluate the effects of different concentrations (1–5000 mg L–1) and sizes (20, 40, and 60 nm) of uncoated ZnO NP compared to ionic ZnSO4 (positive control) on common bean (Phaseolus vulgaris) seed germination. The seeds were soaked in ZnO NP aqueous dispersions for 20 min. The ZnO nanoparticles did not affect the germination rate. The 10 mg L–1 40 nm ZnO treatment showed a tendency to increase weight after 5 days (8.26 ± 0.11 g) when compared to the negative control (7.7 ± 0.7 g). However, at 5000 mg L–1 40 nm ZnO NP and ZnSO4 weight was reduced to 7.7 ± 0.8 g and 6.05 ± 0.08 g, respectively. Microprobe X-ray fluorescence showed that most of the Zn absorbed was trapped in the seed coat, while a small fraction entered the cotyledon. X-ray absorption spectroscopy indicated the biotransformation of the ZnO NP. In the hilum and cotyledon, Zn was found associated with organic molecules such as citrate, malate, and histidine-like compounds. Seedling weight reduction depended on the concentration of Zn taken up by the tissue and on the biotransformation of ZnO into organically bound Zn. Considering the properties of the studied NP, in particular the slow Zn release and lower toxicity compared to ZnSO4, the results represent a step forward toward the application of ZnO NP as an agrochemical.

AB - There is still little information on the potential use of nanomaterials for seed nutrient enhancement through seed priming. The use of nanoparticles (NPs) in agriculture is promising, but in-depth knowledge on their interaction with plants is required. The aim of this study was to evaluate the effects of different concentrations (1–5000 mg L–1) and sizes (20, 40, and 60 nm) of uncoated ZnO NP compared to ionic ZnSO4 (positive control) on common bean (Phaseolus vulgaris) seed germination. The seeds were soaked in ZnO NP aqueous dispersions for 20 min. The ZnO nanoparticles did not affect the germination rate. The 10 mg L–1 40 nm ZnO treatment showed a tendency to increase weight after 5 days (8.26 ± 0.11 g) when compared to the negative control (7.7 ± 0.7 g). However, at 5000 mg L–1 40 nm ZnO NP and ZnSO4 weight was reduced to 7.7 ± 0.8 g and 6.05 ± 0.08 g, respectively. Microprobe X-ray fluorescence showed that most of the Zn absorbed was trapped in the seed coat, while a small fraction entered the cotyledon. X-ray absorption spectroscopy indicated the biotransformation of the ZnO NP. In the hilum and cotyledon, Zn was found associated with organic molecules such as citrate, malate, and histidine-like compounds. Seedling weight reduction depended on the concentration of Zn taken up by the tissue and on the biotransformation of ZnO into organically bound Zn. Considering the properties of the studied NP, in particular the slow Zn release and lower toxicity compared to ZnSO4, the results represent a step forward toward the application of ZnO NP as an agrochemical.

U2 - 10.1021/acsanm.8b01619

DO - 10.1021/acsanm.8b01619

M3 - Article

VL - 1

SP - 6414

EP - 6426

JO - ACS Applied Nano Materials

JF - ACS Applied Nano Materials

SN - 2574-0970

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