Electron-capture detection in reversed-phase liquid chromatography using packed-capillary columns.

B.N. Zegers, J.F.C. de Brouwer, A. Poppema, H. Lingeman, U.A.T. Brinkman

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A new type of interface has been developed for the direct coupling of packed-capillary reversed-phase liquid chromatography with electron-capture detection. This interface allows the detection of relatively non-volatile and/or polar compounds, and simply consists of a fused-silica capillary (50 μm I.D.) inside of a stainless-steel capillary (0.32 mm I.D.) connecting the analytical column with the detector through the oven wall. The make-up gas is used for cooling of the fused-silica capillary. With flow-injection analysis the influence of several parameters (e.g. flow-rate of the make-up gas and mobile phase, oven temperature) on the performance of the total system (i.e. band broadening, sensitivity) has been studied. Using optimal conditions (flow-rate 2 μl min
Original languageEnglish
Pages (from-to)47
JournalAnalytica Chimica Acta
Volume304
DOIs
Publication statusPublished - 1995

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