TY - JOUR
T1 - Electron microscopy of bone
AU - Everts, V.
AU - Hoeben, K.A.
PY - 2013
Y1 - 2013
N2 - Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young animals, are rather simple to prepare and sectioning is not that much of a problem. In the present chapter we describe step-by-step how to prepare mineralized tissues for ultrastructural examination.
AB - Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young animals, are rather simple to prepare and sectioning is not that much of a problem. In the present chapter we describe step-by-step how to prepare mineralized tissues for ultrastructural examination.
U2 - 10.1016/B978-0-12-407239-8.00004-5
DO - 10.1016/B978-0-12-407239-8.00004-5
M3 - Article
SN - 0091-679X
VL - 113
SP - 59
EP - 79
JO - Methods in Cell Biology
JF - Methods in Cell Biology
ER -