Enchytraeus albidus microarray: Enrichment, design, annotation and database (EnchyBASE).

S.C. Novais, J. Arrais, P. Lopes, T. Vandenbrouck, W. de Coen, D. Roelofs, A.M.V.M. Soares, M.J.B. Amorim

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Abstract

Enchytraeus albidus (Oligochaeta) is an ecologically relevant species used as standard test organisms for risk assessment. Effects of stressors in this species are commonly determined at the population level using reproduction and survival as endpoints. The assessment of transcriptomic responses can be very useful e.g. to understand underlying mechanisms of toxicity with gene expression fingerprinting. In the present paper the following is being addressed: 1) development of suppressive subtractive hybridization (SSH) libraries enriched for differentially expressed genes after metal and pesticide exposures; 2) sequencing and characterization of all generated cDNA inserts; 3) development of a publicly available genomic database on E. albidus. A total of 2100 Expressed Sequence Tags (ESTs) were isolated, sequenced and assembled into 1124 clusters (947 singletons and 177 contigs). From these sequences, 41% matched known proteins in GenBank (BLASTX, e-value≤10
Original languageEnglish
Pages (from-to)e34266
JournalPLoS ONE
Volume7 (4)
DOIs
Publication statusPublished - 2012

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