Abstract
Liver injury triggers adaptive remodeling of the hepatic transcriptome for repair/regeneration. We demonstrate that this involves particularly profound transcriptomic alterations where acute induction of genes involved in handling of endoplasmic reticulum stress (ERS) is accompanied by partial hepatic dedifferentiation. Importantly, widespread hepatic gene downregulation could not simply be ascribed to cofactor squelching secondary to ERS gene induction, but rather involves a combination of active repressive mechanisms. ERS acts through inhibition of the liver-identity (LIVER-ID) transcription factor (TF) network, initiated by rapid LIVER-ID TF protein loss. In addition, induction of the transcriptional repressor NFIL3 further contributes to LIVER-ID gene repression. Alteration to the liver TF repertoire translates into compromised activity of regulatory regions characterized by the densest co-recruitment of LIVER-ID TFs and decommissioning of BRD4 super-enhancers driving hepatic identity. While transient repression of the hepatic molecular identity is an intrinsic part of liver repair, sustained disequilibrium between the ERS and LIVER-ID transcriptional programs is linked to liver dysfunction as shown using mouse models of acute liver injury and livers from deceased human septic patients.
Original language | English |
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Article number | e9156 |
Journal | Molecular Systems Biology |
Volume | 16 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 May 2020 |
Funding
The authors acknowledge O. Molendi-Coste, A. Grenon, F. Firmin, C. Mazuy, X. Mar?chal, A. Berthier, D. Sanchez-Lopez, O. Briand, B. Derudas, J. Vandel, L. L'homme (INSERM U1011, Lille, France), Lieselotte Thoolen (Amsterdam Animal Research Center, VU University Amsterdam, The Netherlands), and Shwu-Yuan Wu (University of Texas Southwestern Medical Center, Dallas, USA) for technical assistance and J. Dubois for administrative support. The authors also thank Lei Yin (University of Michigan Medical School, USA), L. Dubuquoy (INSERM U995, Lille, France), and Lies Langouche (KU Leuven, Belgium) for helpful discussions. This work was supported by grants from the Fondation pour la Recherche M?dicale (Equipe labellis?e, DEQ20150331724), ?European Genomic Institute for Diabetes? (E.G.I.D., ANR-10-LABX-46) and European Commission. B.S. is supported by the European Research Council (ERC Grant Immunobile, contract 694717). C-M.C. is supported by US National Institutes of Health (NIH 1RO1CA251698-01), Cancer Prevention & Research Institute of Texas (CPRIT RP180349 and RP190077), and Welch Foundation (I-1805).
Funders | Funder number |
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National Institutes of Health | |
National Cancer Institute | R01CA251698 |
Welch Foundation | I‐1805 |
Cancer Prevention and Research Institute of Texas | RP180349, RP190077 |
University of Texas Southwestern Medical Center | |
Medical School, University of Michigan | U995 |
European Commission | |
European Research Council | 694717 |
Institut national de la santé et de la recherche médicale | U1011 |
Vrije Universiteit Amsterdam | |
Fondation pour la Recherche Médicale | DEQ20150331724, ANR‐10‐LABX‐46 |
Keywords
- liver injury
- NFIL3
- PAR-bZIP
- sepsis
- super-enhancer