Energy transfer and trapping in Synechococcus WH 7803

Alonso M. Acuña, Claire Lemaire, Rienk van Grondelle, Bruno Robert, Ivo H.M. van Stokkum

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Excitation energy transfer (EET) and trapping in Synechococcus WH 7803 whole cells and isolated photosystem I (PSI) complexes have been studied by time-resolved emission spectroscopy at room temperature (RT) and at 77 K. With the help of global and target analysis, the pathways of EET and the charge separation dynamics have been identified. Energy absorbed in the phycobilisome (PB) rods by the abundant phycoerythrin (PE) is funneled to phycocyanin (PC645) and from there to the core that contains allophycocyanin (APC660 and APC680). Intra-PB EET rates have been estimated to range from 11 to 68/ns. It was estimated that at RT, the terminal emitter of the phycobilisome, APC680, transfers its energy at a rate of 90/ns to PSI and at a rate of 50/ns to PSII. At 77 K, the redshifted Chl a states in the PSI core were heterogeneous, with maximum emission at 697 and 707 nm. In 72% of the PSI complexes, the bulk Chl a in equilibrium with F697 decayed with a main trapping lifetime of 39 ps.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalPhotosynthesis Research
Volume135
Issue number1-3
Early online date13 Oct 2017
DOIs
Publication statusPublished - Mar 2018

Keywords

  • Excitation energy transfer
  • Global analysis
  • Light harvesting
  • Target analysis

Cite this

Acuña, Alonso M. ; Lemaire, Claire ; van Grondelle, Rienk ; Robert, Bruno ; van Stokkum, Ivo H.M. / Energy transfer and trapping in Synechococcus WH 7803. In: Photosynthesis Research. 2018 ; Vol. 135, No. 1-3. pp. 115-124.
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abstract = "Excitation energy transfer (EET) and trapping in Synechococcus WH 7803 whole cells and isolated photosystem I (PSI) complexes have been studied by time-resolved emission spectroscopy at room temperature (RT) and at 77 K. With the help of global and target analysis, the pathways of EET and the charge separation dynamics have been identified. Energy absorbed in the phycobilisome (PB) rods by the abundant phycoerythrin (PE) is funneled to phycocyanin (PC645) and from there to the core that contains allophycocyanin (APC660 and APC680). Intra-PB EET rates have been estimated to range from 11 to 68/ns. It was estimated that at RT, the terminal emitter of the phycobilisome, APC680, transfers its energy at a rate of 90/ns to PSI and at a rate of 50/ns to PSII. At 77 K, the redshifted Chl a states in the PSI core were heterogeneous, with maximum emission at 697 and 707 nm. In 72{\%} of the PSI complexes, the bulk Chl a in equilibrium with F697 decayed with a main trapping lifetime of 39 ps.",
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Energy transfer and trapping in Synechococcus WH 7803. / Acuña, Alonso M.; Lemaire, Claire; van Grondelle, Rienk; Robert, Bruno; van Stokkum, Ivo H.M.

In: Photosynthesis Research, Vol. 135, No. 1-3, 03.2018, p. 115-124.

Research output: Contribution to JournalArticleAcademicpeer-review

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