Abstract
Excitation energy transfer (EET) and trapping in Synechococcus WH 7803 whole cells and isolated photosystem I (PSI) complexes have been studied by time-resolved emission spectroscopy at room temperature (RT) and at 77 K. With the help of global and target analysis, the pathways of EET and the charge separation dynamics have been identified. Energy absorbed in the phycobilisome (PB) rods by the abundant phycoerythrin (PE) is funneled to phycocyanin (PC645) and from there to the core that contains allophycocyanin (APC660 and APC680). Intra-PB EET rates have been estimated to range from 11 to 68/ns. It was estimated that at RT, the terminal emitter of the phycobilisome, APC680, transfers its energy at a rate of 90/ns to PSI and at a rate of 50/ns to PSII. At 77 K, the redshifted Chl a states in the PSI core were heterogeneous, with maximum emission at 697 and 707 nm. In 72% of the PSI complexes, the bulk Chl a in equilibrium with F697 decayed with a main trapping lifetime of 39 ps.
| Original language | English |
|---|---|
| Pages (from-to) | 115-124 |
| Number of pages | 10 |
| Journal | Photosynthesis Research |
| Volume | 135 |
| Issue number | 1-3 |
| Early online date | 13 Oct 2017 |
| DOIs | |
| Publication status | Published - Mar 2018 |
Keywords
- Excitation energy transfer
- Global analysis
- Light harvesting
- Target analysis
Cite this
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Energy transfer and trapping in Synechococcus WH 7803. / Acuña, Alonso M.; Lemaire, Claire; van Grondelle, Rienk; Robert, Bruno; van Stokkum, Ivo H.M.
In: Photosynthesis Research, Vol. 135, No. 1-3, 03.2018, p. 115-124.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Energy transfer and trapping in Synechococcus WH 7803
AU - Acuña, Alonso M.
AU - Lemaire, Claire
AU - van Grondelle, Rienk
AU - Robert, Bruno
AU - van Stokkum, Ivo H.M.
PY - 2018/3
Y1 - 2018/3
N2 - Excitation energy transfer (EET) and trapping in Synechococcus WH 7803 whole cells and isolated photosystem I (PSI) complexes have been studied by time-resolved emission spectroscopy at room temperature (RT) and at 77 K. With the help of global and target analysis, the pathways of EET and the charge separation dynamics have been identified. Energy absorbed in the phycobilisome (PB) rods by the abundant phycoerythrin (PE) is funneled to phycocyanin (PC645) and from there to the core that contains allophycocyanin (APC660 and APC680). Intra-PB EET rates have been estimated to range from 11 to 68/ns. It was estimated that at RT, the terminal emitter of the phycobilisome, APC680, transfers its energy at a rate of 90/ns to PSI and at a rate of 50/ns to PSII. At 77 K, the redshifted Chl a states in the PSI core were heterogeneous, with maximum emission at 697 and 707 nm. In 72% of the PSI complexes, the bulk Chl a in equilibrium with F697 decayed with a main trapping lifetime of 39 ps.
AB - Excitation energy transfer (EET) and trapping in Synechococcus WH 7803 whole cells and isolated photosystem I (PSI) complexes have been studied by time-resolved emission spectroscopy at room temperature (RT) and at 77 K. With the help of global and target analysis, the pathways of EET and the charge separation dynamics have been identified. Energy absorbed in the phycobilisome (PB) rods by the abundant phycoerythrin (PE) is funneled to phycocyanin (PC645) and from there to the core that contains allophycocyanin (APC660 and APC680). Intra-PB EET rates have been estimated to range from 11 to 68/ns. It was estimated that at RT, the terminal emitter of the phycobilisome, APC680, transfers its energy at a rate of 90/ns to PSI and at a rate of 50/ns to PSII. At 77 K, the redshifted Chl a states in the PSI core were heterogeneous, with maximum emission at 697 and 707 nm. In 72% of the PSI complexes, the bulk Chl a in equilibrium with F697 decayed with a main trapping lifetime of 39 ps.
KW - Excitation energy transfer
KW - Global analysis
KW - Light harvesting
KW - Target analysis
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U2 - 10.1007/s11120-017-0451-2
DO - 10.1007/s11120-017-0451-2
M3 - Article
VL - 135
SP - 115
EP - 124
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1-3
ER -