Energy transfer and trapping in Synechococcus WH 7803

Alonso M. Acuña, Claire Lemaire, Rienk van Grondelle, Bruno Robert, Ivo H.M. van Stokkum*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review


Excitation energy transfer (EET) and trapping in Synechococcus WH 7803 whole cells and isolated photosystem I (PSI) complexes have been studied by time-resolved emission spectroscopy at room temperature (RT) and at 77 K. With the help of global and target analysis, the pathways of EET and the charge separation dynamics have been identified. Energy absorbed in the phycobilisome (PB) rods by the abundant phycoerythrin (PE) is funneled to phycocyanin (PC645) and from there to the core that contains allophycocyanin (APC660 and APC680). Intra-PB EET rates have been estimated to range from 11 to 68/ns. It was estimated that at RT, the terminal emitter of the phycobilisome, APC680, transfers its energy at a rate of 90/ns to PSI and at a rate of 50/ns to PSII. At 77 K, the redshifted Chl a states in the PSI core were heterogeneous, with maximum emission at 697 and 707 nm. In 72% of the PSI complexes, the bulk Chl a in equilibrium with F697 decayed with a main trapping lifetime of 39 ps.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalPhotosynthesis Research
Issue number1-3
Early online date13 Oct 2017
Publication statusPublished - Mar 2018


Acknowledgements This research was performed as part of the Bio-Solar Cells research programme, sponsored by the Dutch Ministry of Economic Affairs. This work is part of the research program of the Netherlands Organization for Scientific Research (NWO, previously FOM). BR acknowledges funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no 654148 Laserlab-Europe, project LLAMS002152. IHMvS, BR and RvG acknowledge financial support of the European Research Council (Advanced Grant proposal 267333 (PHOTPROT) to RvG and BR).

FundersFunder number
Dutch Ministry of Economic Affairs
Netherlands Organization for Scientific Research
Horizon 2020 Framework ProgrammeLLAMS002152
Seventh Framework Programme267333, 654148
European Research Council
Nederlandse Organisatie voor Wetenschappelijk Onderzoek


    • Excitation energy transfer
    • Global analysis
    • Light harvesting
    • Target analysis


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