Energy transfer from phycobilisomes to photosystem I at room temperature

Avratanu Biswas; Parveen Akhtar; Petar H. Lambrev; Ivo H.M. van Stokkum

doi:10.3389/fpls.2023.1300532

Energy transfer from phycobilisomes to photosystem I at room temperature

Avratanu Biswas, Parveen Akhtar, Petar H. Lambrev, Ivo H.M. van Stokkum^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The phycobilisomes function as the primary light-harvesting antennae in cyanobacteria and red algae, effectively harvesting and transferring excitation energy to both photosystems. Here we investigate the direct energy transfer route from the phycobilisomes to photosystem I at room temperature in a mutant of the cyanobacterium Synechocystis sp. PCC 6803 that lacks photosystem II. The excitation dynamics are studied by picosecond time-resolved fluorescence measurements in combination with global and target analysis. Global analysis revealed several fast equilibration time scales and a decay of the equilibrated system with a time constant of ≈220 ps. From simultaneous target analysis of measurements with two different excitations of 400 nm (chlorophyll a) and 580 nm (phycobilisomes) a transfer rate of 42 ns^-1 from the terminal emitter of the phycobilisome to photosystem I was estimated.

Original language	English
Article number	1300532
Pages (from-to)	1-10
Number of pages	10
Journal	Frontiers in Plant Science
Volume	14
DOIs	https://doi.org/10.3389/fpls.2023.1300532
Publication status	Published - 2023

Bibliographical note

Published online: 8 January 2024.

Publisher Copyright:
Copyright © 2024 Biswas, Akhtar, Lambrev and van Stokkum.

Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grants from the National Research, Development and Innovation Fund (NKFI FK-139067 to PA and ANN-144012 and 2018-1.2.1-NKP-2018-00009 to PL) and the Hungarian Research Network (SA-76/2021 to PA). The research leading to these results has received funding from LASERLAB-EUROPE (grant agreement no. 871124, European Union’s Horizon 2020 research and innovation program). Acknowledgments

Funders	Funder number
Nemzeti Kutatási, Fejlesztési és Innovaciós Alap
Hungarian Research Network	SA-76/2021
Nemzeti Kutatási Fejlesztési és Innovációs Hivatal	2018-1.2.1-NKP-2018-00009, ANN-144012, FK-139067
Horizon 2020 Framework Programme	871124

Keywords

energy transfer (ET)
global analysis (GA)
photosystem I (PSI)
phycobilisomes (PBs)
target analysis

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10.3389/fpls.2023.1300532

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title = "Energy transfer from phycobilisomes to photosystem I at room temperature",

abstract = "The phycobilisomes function as the primary light-harvesting antennae in cyanobacteria and red algae, effectively harvesting and transferring excitation energy to both photosystems. Here we investigate the direct energy transfer route from the phycobilisomes to photosystem I at room temperature in a mutant of the cyanobacterium Synechocystis sp. PCC 6803 that lacks photosystem II. The excitation dynamics are studied by picosecond time-resolved fluorescence measurements in combination with global and target analysis. Global analysis revealed several fast equilibration time scales and a decay of the equilibrated system with a time constant of ≈220 ps. From simultaneous target analysis of measurements with two different excitations of 400 nm (chlorophyll a) and 580 nm (phycobilisomes) a transfer rate of 42 ns-1 from the terminal emitter of the phycobilisome to photosystem I was estimated.",

keywords = "energy transfer (ET), global analysis (GA), photosystem I (PSI), phycobilisomes (PBs), target analysis",

author = "Avratanu Biswas and Parveen Akhtar and Lambrev, \{Petar H.\} and \{van Stokkum\}, \{Ivo H.M.\}",

note = "Published online: 8 January 2024. Publisher Copyright: Copyright {\textcopyright} 2024 Biswas, Akhtar, Lambrev and van Stokkum.",

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AU - Biswas, Avratanu

AU - Akhtar, Parveen

AU - Lambrev, Petar H.

AU - van Stokkum, Ivo H.M.

PY - 2023

Y1 - 2023

N2 - The phycobilisomes function as the primary light-harvesting antennae in cyanobacteria and red algae, effectively harvesting and transferring excitation energy to both photosystems. Here we investigate the direct energy transfer route from the phycobilisomes to photosystem I at room temperature in a mutant of the cyanobacterium Synechocystis sp. PCC 6803 that lacks photosystem II. The excitation dynamics are studied by picosecond time-resolved fluorescence measurements in combination with global and target analysis. Global analysis revealed several fast equilibration time scales and a decay of the equilibrated system with a time constant of ≈220 ps. From simultaneous target analysis of measurements with two different excitations of 400 nm (chlorophyll a) and 580 nm (phycobilisomes) a transfer rate of 42 ns-1 from the terminal emitter of the phycobilisome to photosystem I was estimated.

AB - The phycobilisomes function as the primary light-harvesting antennae in cyanobacteria and red algae, effectively harvesting and transferring excitation energy to both photosystems. Here we investigate the direct energy transfer route from the phycobilisomes to photosystem I at room temperature in a mutant of the cyanobacterium Synechocystis sp. PCC 6803 that lacks photosystem II. The excitation dynamics are studied by picosecond time-resolved fluorescence measurements in combination with global and target analysis. Global analysis revealed several fast equilibration time scales and a decay of the equilibrated system with a time constant of ≈220 ps. From simultaneous target analysis of measurements with two different excitations of 400 nm (chlorophyll a) and 580 nm (phycobilisomes) a transfer rate of 42 ns-1 from the terminal emitter of the phycobilisome to photosystem I was estimated.

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KW - global analysis (GA)

KW - photosystem I (PSI)

KW - phycobilisomes (PBs)

KW - target analysis

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ER -

Energy transfer from phycobilisomes to photosystem I at room temperature

Abstract

Bibliographical note

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Keywords

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