TY - JOUR
T1 - Engram-specific transcriptome profiling of contextual memory consolidation
AU - Rao-Ruiz, Priyanka
AU - Couey, Jonathan J.
AU - Marcelo, Ivo M.
AU - Bouwkamp, Christian G.
AU - Slump, Denise E.
AU - Matos, Mariana R.
AU - van der Loo, Rolinka J.
AU - Martins, Gabriela J.
AU - van den Hout, Mirjam
AU - van IJcken, Wilfred F.
AU - Costa, Rui M.
AU - van den Oever, Michel C.
AU - Kushner, Steven A.
PY - 2019/12/1
Y1 - 2019/12/1
N2 -
Sparse populations of neurons in the dentate gyrus (DG) of the hippocampus are causally implicated in the encoding of contextual fear memories. However, engram-specific molecular mechanisms underlying memory consolidation remain largely unknown. Here we perform unbiased RNA sequencing of DG engram neurons 24 h after contextual fear conditioning to identify transcriptome changes specific to memory consolidation. DG engram neurons exhibit a highly distinct pattern of gene expression, in which CREB-dependent transcription features prominently (P = 6.2 × 10
−13
), including Atf3 (P = 2.4 × 10
−41
), Penk (P = 1.3 × 10
−15
), and Kcnq3 (P = 3.1 × 10
−12
). Moreover, we validate the functional relevance of the RNAseq findings by establishing the causal requirement of intact CREB function specifically within the DG engram during memory consolidation, and identify a novel group of CREB target genes involved in the encoding of long-term memory.
AB -
Sparse populations of neurons in the dentate gyrus (DG) of the hippocampus are causally implicated in the encoding of contextual fear memories. However, engram-specific molecular mechanisms underlying memory consolidation remain largely unknown. Here we perform unbiased RNA sequencing of DG engram neurons 24 h after contextual fear conditioning to identify transcriptome changes specific to memory consolidation. DG engram neurons exhibit a highly distinct pattern of gene expression, in which CREB-dependent transcription features prominently (P = 6.2 × 10
−13
), including Atf3 (P = 2.4 × 10
−41
), Penk (P = 1.3 × 10
−15
), and Kcnq3 (P = 3.1 × 10
−12
). Moreover, we validate the functional relevance of the RNAseq findings by establishing the causal requirement of intact CREB function specifically within the DG engram during memory consolidation, and identify a novel group of CREB target genes involved in the encoding of long-term memory.
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U2 - 10.1038/s41467-019-09960-x
DO - 10.1038/s41467-019-09960-x
M3 - Article
AN - SCOPUS:85066022034
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2232
ER -