Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

I. V. Fedotov; L. V. Doronina-Amitonova; D. A. Sidorov-Biryukov; A. B. Fedotov; K. V. Anokhin; S. Ya Kilin; K. Sakoda; A. M. Zheltikov

doi:10.1063/1.4864646

Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

I. V. Fedotov, L. V. Doronina-Amitonova, D. A. Sidorov-Biryukov, A. B. Fedotov, K. V. Anokhin, S. Ya Kilin, K. Sakoda, A. M. Zheltikov

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen-vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser-fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

Original language	English
Article number	83702
Journal	Applied Physics Letters
Volume	104
Issue number	8
DOIs	https://doi.org/10.1063/1.4864646
Publication status	Published - 1 Jan 2014
Externally published	Yes

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title = "Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation",

abstract = "Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen-vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser-fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.",

author = "Fedotov, {I. V.} and Doronina-Amitonova, {L. V.} and Sidorov-Biryukov, {D. A.} and Fedotov, {A. B.} and Anokhin, {K. V.} and Kilin, {S. Ya} and K. Sakoda and Zheltikov, {A. M.}",

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doi = "10.1063/1.4864646",

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AU - Fedotov, I. V.

AU - Doronina-Amitonova, L. V.

AU - Sidorov-Biryukov, D. A.

AU - Fedotov, A. B.

AU - Anokhin, K. V.

AU - Kilin, S. Ya

AU - Sakoda, K.

AU - Zheltikov, A. M.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen-vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser-fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

AB - Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen-vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser-fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

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Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

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