Abstract
Plastic particles have been proven to be abundant in the aquatic environment, raising concerns about their potential toxic effects. In the present study, we determined the bioaccumulation potential of bisphenol A (BPA) in adult zebrafish (Danio rerio) in the absence and presence of nano-sized plastic particles (nanoplastics, NPPs). Results show that BPA can accumulate in the viscera, gill, head and muscle of zebrafish with 85, 43, 20, and 3 μg/g ww after 1 d exposure. NPPs were also found to accumulate in different tissues of the fish. Relative equilibrium was reached after 1 d exposure in different tissues with 39 to 636 mg/kg ww. Co-exposure of NPPs and BPA led to a 2.2 and 2.6-fold significant increment of BPA uptake in the head and viscera, if compared with BPA alone treatment after 3 d exposure. As such, we further investigated several neurotoxic biomarker alterations in the fish head. It was found that either BPA or NPPs can cause myelin basic protein (MBP)/gene up-regulation in the central nervous system (CNS); meanwhile, both contaminants exhibited significant inhibition of acetylcholinesterase (AChE) activity, which is a well-known representative biomarker for neurotoxicity. Moreover, for the co-exposure treatment, biomarkers of myeline and tubulin protein/gene expressions, dopamine content, and the mRNA expression of mesencephalic astrocyte derived neurotrophic factor (MANF) were all significantly up-regulated, suggesting that an enhanced neurotoxic effects in both CNS and dopaminergic system occurred. However, AChE activity was no more inhibited in the co-exposure treatment, which implies that solely AChE measurement may not be sufficient to identify neurotoxic effects in the cholinergic system. Overall, the present study demonstrates that the presence of NPPs can increase BPA bioavailability and cause neurotoxicity in adult zebrafish.
Original language | English |
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Pages (from-to) | 1312-1321 |
Number of pages | 10 |
Journal | Science of the Total Environment |
Volume | 609 |
Issue number | dec 2017 |
DOIs | |
Publication status | Published - 7 Aug 2017 |
Funding
This work was supported by the National Natural Science Foundation of China (21577104; 21477086) and the China Postdoctoral Science Foundation Grant (2015M581657). The research stay of Qiqing Chen at Institute for Environmental Research, RWTH Aachen University, Germany was supported by the CSC-DAAD postdoctoral scholarship.
Funders | Funder number |
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National Natural Science Foundation of China | 21477086, 21577104 |
China Postdoctoral Science Foundation | 2015M581657 |