Ensemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia

Jona Mijalkovic, B. Prevo, Felix Oswald, P.J.J. Mangeol, Erwin J G Peterman

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Cytoplasmic dyneins drive microtubule-based, minus-end directed transport in eukaryotic cells. Whereas cytoplasmic dynein 1 has been widely studied, IFT dynein has received far less attention. Here, we use fluorescence microscopy of labelled motors in living Caenorhabditis elegans to investigate IFT-dynein motility at the ensemble and single-molecule level. We find that while the kinesin composition of motor ensembles varies along the track, the amount of dynein remains relatively constant. Remarkably, this does not result in directionality changes of cargo along the track, as has been reported for other opposite-polarity, tug-of-war motility systems. At the single-molecule level, IFT-dynein trajectories reveal unexpected dynamics, including diffusion at the base, and pausing and directional switches along the cilium. Stochastic simulations show that the ensemble IFT-dynein distribution depends upon the probability of single-motor directional switches. Our results provide quantitative insight into IFT-dynein dynamics in vivo, shedding light on the complex functioning of dynein motors in general.

Original languageEnglish
Article number14591
Pages (from-to)14591
JournalNature Communications
Volume8
DOIs
Publication statusPublished - 23 Feb 2017

Keywords

  • Journal Article

Fingerprint

Dive into the research topics of 'Ensemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia'. Together they form a unique fingerprint.

Cite this