Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains

Salma A. Shoulah; Anna M. Oschmann; Abdelfattah Selim; Torsten Semmler; Carsten Schwarz; Elisabeth Kamal; Faysal Hamouda; Elsayed Galila; Wilbert Bitter; Astrid Lewin

doi:10.1016/j.plasmid.2018.01.003

Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains

Salma A. Shoulah, Anna M. Oschmann, Abdelfattah Selim, Torsten Semmler, Carsten Schwarz, Elisabeth Kamal, Faysal Hamouda, Elsayed Galila, Wilbert Bitter, Astrid Lewin^*

^*Corresponding author for this work

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Abstract

Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of 47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.

Original language	English
Pages (from-to)	28-35
Number of pages	8
Journal	Plasmid
Volume	95
Early online date	16 Jan 2018
DOIs	https://doi.org/10.1016/j.plasmid.2018.01.003
Publication status	Published - Jan 2018

Funding

We thank Dr. Elvira Richter ( National Reference Center for Mycobacteria , Borstel, Germany) for providing the MAH clinical isolates, Roland Schulze-Röbbecke ( University Hospital Düsseldorf , Germany) for providing water isolates of MAH and Petra Möbius ( Friedrich Löffler Institute , Jena, Germany) for providing a soil isolate from MAH. We further thank Dr. Lei Mao from the Bioinformatics Support of the Robert Koch Institute (MF1) for her support in bioinformatics and statistical analyses, and Andrea Sanchini for his help with gene function analysis. Salma Shoulah was supported by Ministry of Higher Education and Scientific Research in Egypt.

Funders	Funder number
Ministry of Higher Education and Scientific Research
Ministry of Higher Education and Scientific Research

Keywords

Antibiotic resistance
Conjugation
Mycobacterium avium
Plasmid
Type I restriction modification

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.plasmid.2018.01.003

Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strainsFinal published version, 1.52 MBLicence: Article 25fa Dutch Copyright Act

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title = "Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains",

abstract = "Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of 47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.",

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T1 - Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains

AU - Shoulah, Salma A.

AU - Oschmann, Anna M.

AU - Selim, Abdelfattah

AU - Semmler, Torsten

AU - Schwarz, Carsten

AU - Kamal, Elisabeth

AU - Hamouda, Faysal

AU - Galila, Elsayed

AU - Bitter, Wilbert

AU - Lewin, Astrid

PY - 2018/1

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N2 - Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of 47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.

AB - Mycobacterium avium subsp. hominissuis (MAH) is a widespread opportunistic pathogen that can be isolated from environment (dust, soil and water) and patients with lung or lymphnode infection. In our previous research we revealed the pronounced genetic diversity in MAH by identifying eight different types of a newly described genomic island. In order to identify mechanisms of such horizontal gene transfer we now analyzed the ability of 47 MAH isolates to inherit the conjugative plasmid pRAW from M. marinum. A higher percentage of environmental isolates (22.7%) compared to clinical isolates (8%) had the capacity to function as recipient in conjugal plasmid transfer. Genetic analysis showed additionally that environmental isolates contained more genes homologous to genes present on conjugative mycobacterial plasmids than clinical isolates. Comparative analysis of the genomes of the isolates pointed to a possible association between the ability to act as recipient in conjugation and the structure of a genomic region containing the radC gene and a type I restriction/modification system. Finally we found that uptake of pRAW decreased the resistance against various antibiotics.

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KW - Type I restriction modification

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Environmental Mycobacterium avium subsp. hominissuis have a higher probability to act as a recipient in conjugation than clinical strains

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UN SDGs

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