Enzyme activation and catalysis: characterisation of the vibrational modes of substrate and product in protochlorophyllide oxidoreductase

O. Sytina, M.T. Alexandre, D.J. Heyes, C.N. Hunter, B. Robert, R. van Grondelle, M.L. Groot

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The light-dependent reduction of protochlorophyllide, a key step in the synthesis of chlorophyll, is catalyzed by the enzyme protochlorophyllide oxidoreductase (POR) and requires two photons (O. A. Sytina et al., Nature, 2008, 456, 1001-1008). The first photon activates the enzyme-substrate complex, a subsequent second photon initiates the photochemistry by triggering the formation of a catalytic intermediate. These two events are characterized by different spectral changes in the infra-red spectral region. Here, we investigate the vibrational frequencies of the POR-bound and unbound substrate, and product, and thus provide a detailed assignment of the spectral changes in the 1800-1250 cm
Original languageEnglish
Pages (from-to)2307
Number of pages6
JournalPhysical Chemistry Chemical Physics - PCCP
Issue number6
Publication statusPublished - 2011


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