Epithelial cell detachment by Porphyromonas gingivalis biofilm and planktonic cultures

L. Huang, C. van Loveren, J. Ling, X. Wei, W. Crielaard, D.M. Deng

Research output: Contribution to JournalArticleAcademicpeer-review


Porphyromonas gingivalis is present as a biofilm at the sites of periodontal infections. The detachment of gingival epithelial cells induced by P. gingivalis biofilms was examined using planktonic cultures as a comparison. Exponentially grown planktonic cultures or 40-h biofilms were co-incubated with epithelial cells in a 24-well plate for 4 h. Epithelial cell detachment was assessed using imaging. The activity of arginine-gingipain (Rgp) and gene expression profiles of P. gingivalis cultures were examined using a gingipain assay and quantitative PCR, respectively. P. gingivalis biofilms induced significantly higher cell detachment and displayed higher Rgp activity compared to the planktonic cultures. The genes involved in gingipain post-translational modification, but not rgp genes, were significantly up-regulated in P. gingivalis biofilms. The results underline the importance of including biofilms in the study of bacterial and host cell interactions.
Original languageEnglish
Pages (from-to)489-496
Issue number4
Publication statusPublished - 2016


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