Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 3959-3968 |
| Journal | Applied and Environmental Microbiology |
| Volume | 74 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 2008 |
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Eukaryotic diversity in an anaerobic aquifer polluted with landfill leachate. / Brad, T.; Braster, M.; van Breukelen, B.M.; van Straalen, N.M.; Roling, W.F.M.
In: Applied and Environmental Microbiology, Vol. 74, No. 13, 2008, p. 3959-3968.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Eukaryotic diversity in an anaerobic aquifer polluted with landfill leachate
AU - Brad, T.
AU - Braster, M.
AU - van Breukelen, B.M.
AU - van Straalen, N.M.
AU - Roling, W.F.M.
PY - 2008
Y1 - 2008
N2 - Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sediment-associated eukaryotic community composition in an anaerobic sandy aquifer polluted with landfill leachate (Banisveld, The Netherlands). The microeukaryotic community at a depth of 1 to 5 m below the surface along a transect downgradient (21 to 68 m) from the landfill and at a clean reference location was diverse. Fungal sequences dominated most clone libraries. The fungal diversity was high, and most sequences were sequences of yeasts of the Basidiomycota. Sequences of green algae (Chlorophyta) were detected in parts of the aquifer close (<30 m) to the landfill. The bacterium-predating nanoflagellate Heteromita globosa (Cercozoa) was retrieved in enrichments, and its sequences dominated the clone library derived from the polluted aquifer at a depth of 5 m at a location 21 m downgradient from the landfill. The number of culturable eukaryotes ranged from 10
AB - Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sediment-associated eukaryotic community composition in an anaerobic sandy aquifer polluted with landfill leachate (Banisveld, The Netherlands). The microeukaryotic community at a depth of 1 to 5 m below the surface along a transect downgradient (21 to 68 m) from the landfill and at a clean reference location was diverse. Fungal sequences dominated most clone libraries. The fungal diversity was high, and most sequences were sequences of yeasts of the Basidiomycota. Sequences of green algae (Chlorophyta) were detected in parts of the aquifer close (<30 m) to the landfill. The bacterium-predating nanoflagellate Heteromita globosa (Cercozoa) was retrieved in enrichments, and its sequences dominated the clone library derived from the polluted aquifer at a depth of 5 m at a location 21 m downgradient from the landfill. The number of culturable eukaryotes ranged from 10
U2 - 10.1128/AEM.02820-07
DO - 10.1128/AEM.02820-07
M3 - Article
VL - 74
SP - 3959
EP - 3968
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 13
ER -