Eukaryotic diversity in an anaerobic aquifer polluted with landfill leachate

T. Brad, M. Braster, B.M. van Breukelen, N.M. van Straalen, W.F.M. Roling

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sediment-associated eukaryotic community composition in an anaerobic sandy aquifer polluted with landfill leachate (Banisveld, The Netherlands). The microeukaryotic community at a depth of 1 to 5 m below the surface along a transect downgradient (21 to 68 m) from the landfill and at a clean reference location was diverse. Fungal sequences dominated most clone libraries. The fungal diversity was high, and most sequences were sequences of yeasts of the Basidiomycota. Sequences of green algae (Chlorophyta) were detected in parts of the aquifer close (<30 m) to the landfill. The bacterium-predating nanoflagellate Heteromita globosa (Cercozoa) was retrieved in enrichments, and its sequences dominated the clone library derived from the polluted aquifer at a depth of 5 m at a location 21 m downgradient from the landfill. The number of culturable eukaryotes ranged from 10
Original languageEnglish
Pages (from-to)3959-3968
JournalApplied and Environmental Microbiology
Volume74
Issue number13
DOIs
Publication statusPublished - 2008

Fingerprint

Chemical Water Pollutants
landfill leachates
Groundwater
landfills
Waste Disposal Facilities
aquifers
landfill
eukaryote
aquifer
Chlorophyta
eukaryotic cells
clone
Eukaryota
clones
Cercozoa
bacterium
Clone Cells
bacteria
Bacteria
Basidiomycota

Cite this

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title = "Eukaryotic diversity in an anaerobic aquifer polluted with landfill leachate",
abstract = "Eukaryotes may influence pollutant degradation processes in groundwater ecosystems by activities such as predation on bacteria and recycling of nutrients. Culture-independent community profiling and phylogenetic analysis of 18S rRNA gene fragments, as well as culturing, were employed to obtain insight into the sediment-associated eukaryotic community composition in an anaerobic sandy aquifer polluted with landfill leachate (Banisveld, The Netherlands). The microeukaryotic community at a depth of 1 to 5 m below the surface along a transect downgradient (21 to 68 m) from the landfill and at a clean reference location was diverse. Fungal sequences dominated most clone libraries. The fungal diversity was high, and most sequences were sequences of yeasts of the Basidiomycota. Sequences of green algae (Chlorophyta) were detected in parts of the aquifer close (<30 m) to the landfill. The bacterium-predating nanoflagellate Heteromita globosa (Cercozoa) was retrieved in enrichments, and its sequences dominated the clone library derived from the polluted aquifer at a depth of 5 m at a location 21 m downgradient from the landfill. The number of culturable eukaryotes ranged from 10",
author = "T. Brad and M. Braster and {van Breukelen}, B.M. and {van Straalen}, N.M. and W.F.M. Roling",
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Eukaryotic diversity in an anaerobic aquifer polluted with landfill leachate. / Brad, T.; Braster, M.; van Breukelen, B.M.; van Straalen, N.M.; Roling, W.F.M.

In: Applied and Environmental Microbiology, Vol. 74, No. 13, 2008, p. 3959-3968.

Research output: Contribution to JournalArticleAcademicpeer-review

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