Excited state proton transfer in strongly enhanced GFP (sGFP2)

B.F. van Oort; M.J.T. ter Veer; M.L. Groot; I.H.M. van Stokkum

doi:10.1039/c2cp40694b

Excited state proton transfer in strongly enhanced GFP (sGFP2)

B.F. van Oort, M.J.T. ter Veer, M.L. Groot, I.H.M. van Stokkum

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Proton transfer is an elementary process in biology. Green fluorescent protein (GFP) has served as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. We have used pump-dump-probe spectroscopy to study how proton transfer through the 'proton-wire' around the chromophore is affected by a combination of mutations in a modern GFP variety (sGFP2). The results indicate that in H

Original language	English
Pages (from-to)	8852-8858
Journal	Physical Chemistry Chemical Physics - PCCP
Volume	14
Issue number	25
DOIs	https://doi.org/10.1039/c2cp40694b
Publication status	Published - 2012

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title = "Excited state proton transfer in strongly enhanced GFP (sGFP2)",

abstract = "Proton transfer is an elementary process in biology. Green fluorescent protein (GFP) has served as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. We have used pump-dump-probe spectroscopy to study how proton transfer through the 'proton-wire' around the chromophore is affected by a combination of mutations in a modern GFP variety (sGFP2). The results indicate that in H",

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AU - Groot, M.L.

AU - van Stokkum, I.H.M.

PY - 2012

Y1 - 2012

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AB - Proton transfer is an elementary process in biology. Green fluorescent protein (GFP) has served as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. We have used pump-dump-probe spectroscopy to study how proton transfer through the 'proton-wire' around the chromophore is affected by a combination of mutations in a modern GFP variety (sGFP2). The results indicate that in H

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JO - Physical Chemistry Chemical Physics - PCCP

JF - Physical Chemistry Chemical Physics - PCCP

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Excited state proton transfer in strongly enhanced GFP (sGFP2)

Abstract

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