Excited state proton transfer in strongly enhanced GFP (sGFP2)

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Abstract

Proton transfer is an elementary process in biology. Green fluorescent protein (GFP) has served as an important model system to elucidate the mechanistic details of this reaction, because in GFP proton transfer can be induced by light absorption. We have used pump-dump-probe spectroscopy to study how proton transfer through the 'proton-wire' around the chromophore is affected by a combination of mutations in a modern GFP variety (sGFP2). The results indicate that in H
Original languageEnglish
Pages (from-to)8852-8858
JournalPhysical Chemistry Chemical Physics - PCCP
Volume14
Issue number25
DOIs
Publication statusPublished - 2012

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