Expanding the enzyme universe with genetically encoded unnatural amino acids

Ivana Drienovská; Gerard Roelfes

doi:10.1038/s41929-019-0410-8

Expanding the enzyme universe with genetically encoded unnatural amino acids

Ivana Drienovská^*
, Gerard Roelfes

^*Corresponding author for this work

Research output: Contribution to Journal › Review article › Academic › peer-review

Abstract

The emergence of robust methods to expand the genetic code allows incorporation of non-canonical amino acids into the polypeptide chain of proteins, thus making it possible to introduce unnatural chemical functionalities in enzymes. In this Perspective, we show how this powerful methodology is used to create enzymes with improved and novel, even new-to-nature, catalytic activities. We provide an overview of the current state of the art, and discuss the potential benefits of developing and using enzymes with genetically encoded non-canonical amino acids compared with enzymes containing only canonical amino acids.

Original language	English
Pages (from-to)	193-202
Number of pages	10
Journal	Nature Catalysis
Volume	3
Issue number	3
DOIs	https://doi.org/10.1038/s41929-019-0410-8
Publication status	Published - 1 Mar 2020
Externally published	Yes

Funding

G.R. acknowledges financial support from the Netherlands Organisation for Scientific Research (NWO, Vici grant 724.013.003) and the Ministry of Education Culture and Science (Gravitation programme no. 024.001.035).

Funders	Funder number
Ministry of Education Culture and Science	024.001.035
Ministerie van Onderwijs, Cultuur en Wetenschap
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	724.013.003

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Expanding the enzyme universe with genetically encoded unnatural amino acids

Abstract

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