Extracellular ATP elevates cytoplasmatic free Ca2+ in HeLa cells by the interaction with a 5'-nucleotide receptor

M J Smit; R Leurs; S M Bloemers; L G Tertoolen; A Bast; S W De Laat; H Timmerman

Extracellular ATP elevates cytoplasmatic free Ca2+ in HeLa cells by the interaction with a 5'-nucleotide receptor

M J Smit, R Leurs, S M Bloemers, L G Tertoolen, A Bast, S W De Laat, H Timmerman

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Abstract

In the present study we have characterized the effects of ATP and several other nucleotides on the intracellular Ca2+ levels of HeLa cells. Using fura-2 microscopy fluorescence measurements, the ATP-mediated increase in intracellular Ca2+ was shown to consist of a rapid rise which decreased after a few seconds to a sustained elevated level. In the absence of extracellular Ca2+ or in the presence of 10 microM La3+, only a transient elevation of the Ca2+ concentration was observed. The ATP responses were not altered after treatment of HeLa cells with cholera toxin or pertussis toxin. Pharmacological analysis of this calcium response revealed that this effect was not mediated by the classical P2y purinoceptor but by a 5'-nucleotide receptor.

Original language	English
Pages (from-to)	223-6
Number of pages	4
Journal	European Journal of Pharmacology
Volume	247
Issue number	2
Publication status	Published - 15 Oct 1993

Keywords

Adenosine Triphosphate
Calcium
Cholera Toxin
Cytoplasm
Fura-2
GTP-Binding Proteins
HeLa Cells
Humans
Pertussis Toxin
Receptors, Purinergic P2
Uridine Triphosphate
Virulence Factors, Bordetella
Journal Article

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title = "Extracellular ATP elevates cytoplasmatic free Ca2+ in HeLa cells by the interaction with a 5'-nucleotide receptor",

abstract = "In the present study we have characterized the effects of ATP and several other nucleotides on the intracellular Ca2+ levels of HeLa cells. Using fura-2 microscopy fluorescence measurements, the ATP-mediated increase in intracellular Ca2+ was shown to consist of a rapid rise which decreased after a few seconds to a sustained elevated level. In the absence of extracellular Ca2+ or in the presence of 10 microM La3+, only a transient elevation of the Ca2+ concentration was observed. The ATP responses were not altered after treatment of HeLa cells with cholera toxin or pertussis toxin. Pharmacological analysis of this calcium response revealed that this effect was not mediated by the classical P2y purinoceptor but by a 5'-nucleotide receptor.",

keywords = "Adenosine Triphosphate, Calcium, Cholera Toxin, Cytoplasm, Fura-2, GTP-Binding Proteins, HeLa Cells, Humans, Pertussis Toxin, Receptors, Purinergic P2, Uridine Triphosphate, Virulence Factors, Bordetella, Journal Article",

author = "Smit, {M J} and R Leurs and Bloemers, {S M} and Tertoolen, {L G} and A Bast and {De Laat}, {S W} and H Timmerman",

year = "1993",

month = oct,

day = "15",

language = "English",

volume = "247",

pages = "223--6",

journal = "European Journal of Pharmacology",

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publisher = "Elsevier",

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TY - JOUR

T1 - Extracellular ATP elevates cytoplasmatic free Ca2+ in HeLa cells by the interaction with a 5'-nucleotide receptor

AU - Smit, M J

AU - Leurs, R

AU - Bloemers, S M

AU - Tertoolen, L G

AU - Bast, A

AU - De Laat, S W

AU - Timmerman, H

PY - 1993/10/15

Y1 - 1993/10/15

N2 - In the present study we have characterized the effects of ATP and several other nucleotides on the intracellular Ca2+ levels of HeLa cells. Using fura-2 microscopy fluorescence measurements, the ATP-mediated increase in intracellular Ca2+ was shown to consist of a rapid rise which decreased after a few seconds to a sustained elevated level. In the absence of extracellular Ca2+ or in the presence of 10 microM La3+, only a transient elevation of the Ca2+ concentration was observed. The ATP responses were not altered after treatment of HeLa cells with cholera toxin or pertussis toxin. Pharmacological analysis of this calcium response revealed that this effect was not mediated by the classical P2y purinoceptor but by a 5'-nucleotide receptor.

AB - In the present study we have characterized the effects of ATP and several other nucleotides on the intracellular Ca2+ levels of HeLa cells. Using fura-2 microscopy fluorescence measurements, the ATP-mediated increase in intracellular Ca2+ was shown to consist of a rapid rise which decreased after a few seconds to a sustained elevated level. In the absence of extracellular Ca2+ or in the presence of 10 microM La3+, only a transient elevation of the Ca2+ concentration was observed. The ATP responses were not altered after treatment of HeLa cells with cholera toxin or pertussis toxin. Pharmacological analysis of this calcium response revealed that this effect was not mediated by the classical P2y purinoceptor but by a 5'-nucleotide receptor.

KW - Adenosine Triphosphate

KW - Calcium

KW - Cholera Toxin

KW - Cytoplasm

KW - Fura-2

KW - GTP-Binding Proteins

KW - HeLa Cells

KW - Humans

KW - Pertussis Toxin

KW - Receptors, Purinergic P2

KW - Uridine Triphosphate

KW - Virulence Factors, Bordetella

KW - Journal Article

M3 - Article

C2 - 8282011

SN - 0014-2999

VL - 247

SP - 223

EP - 226

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2

ER -

Extracellular ATP elevates cytoplasmatic free Ca2+ in HeLa cells by the interaction with a 5'-nucleotide receptor

Abstract

Keywords

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