TY - JOUR
T1 - Extracellular production of recombinant proteins using bacterial autotransporters.
AU - Jong, W.S.P.
AU - Sauri, A.
AU - Luirink, S.
PY - 2010
Y1 - 2010
N2 - Escherichia coli is still a very popular host for the production of recombinant proteins at an analytical or industrial scale. Secretion of the proteins into the culture medium or display at the cell surface would be preferred in many applications but is hampered by the complex two-layered cell envelope. The autotransporter pathway is used by E. coli to secrete virulence factors via a relatively simple but efficient and specific mechanism. Here we discuss recent progress in the structural and mechanistic analysis of this pathway and the implications for future development of a versatile platform for secretion and display of heterologous proteins. © 2010 Elsevier Ltd.
AB - Escherichia coli is still a very popular host for the production of recombinant proteins at an analytical or industrial scale. Secretion of the proteins into the culture medium or display at the cell surface would be preferred in many applications but is hampered by the complex two-layered cell envelope. The autotransporter pathway is used by E. coli to secrete virulence factors via a relatively simple but efficient and specific mechanism. Here we discuss recent progress in the structural and mechanistic analysis of this pathway and the implications for future development of a versatile platform for secretion and display of heterologous proteins. © 2010 Elsevier Ltd.
UR - https://www.scopus.com/pages/publications/77957354322
UR - https://www.scopus.com/inward/citedby.url?scp=77957354322&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2010.07.009
DO - 10.1016/j.copbio.2010.07.009
M3 - Article
SN - 0958-1669
VL - 21
SP - 646
EP - 652
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 5
ER -