Fabrication of cell container arrays with overlaid surface topographies

Roman Truckenmüller, Stefan Giselbrecht, Maryana Escalante-Marun, Max Groenendijk, Bernke Papenburg, Nicolas Rivron, Hemant Unadkat, Volker Saile, Vinod Subramaniam, Albert van den Berg, Clemens van Blitterswijk, Matthias Wessling, Jan de Boer, Dimitrios Stamatialis

    Research output: Contribution to JournalArticleAcademicpeer-review


    This paper presents cell culture substrates in the form of microcontainer arrays with overlaid surface topographies, and a technology for their fabrication. The new fabrication technology is based on microscale thermoforming of thin polymer films whose surfaces are topographically prepatterned on a micro- or nanoscale. For microthermoforming, we apply a new process on the basis of temporary back moulding of polymer films and use the novel concept of a perforated-sheet-like mould. Thermal micro- or nanoimprinting is applied for prepatterning. The novel cell container arrays are fabricated from polylactic acid (PLA) films. The thin-walled microcontainer structures have the shape of a spherical calotte merging into a hexagonal shape at their upper circumferential edges. In the arrays, the cell containers are arranged densely packed in honeycomb fashion. The inner surfaces of the highly curved container walls are provided with various topographical micro- and nanopatterns. For a first validation of the microcontainer arrays as in vitro cell culture substrates, C2C12 mouse premyoblasts are cultured in containers with microgrooved surfaces and shown to align along the grooves in the three-dimensional film substrates. In future stem-cell-biological and tissue engineering applications, microcontainers fabricated using the proposed technology may act as geometrically defined artificial microenvironments or niches.

    Original languageEnglish
    Pages (from-to)95-107
    Number of pages13
    JournalBiomedical Microdevices
    Issue number1
    Publication statusPublished - Feb 2012


    • Animals
    • Cell Line
    • High-Throughput Screening Assays
    • Lactic Acid
    • Mice
    • Polyesters
    • Polymers
    • Surface Properties
    • Tissue Engineering
    • Journal Article
    • Research Support, Non-U.S. Gov't


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