Far-red fluorescence: A direct spectroscopic marker for LHCII oligomer formation in non-photochemical quenching

Yuliya Miloslavina*, Antje Wehner, Petar H. Lambrev, Emilie Wientjes, Michael Reus, Gyozo Garab, Roberta Croce, Alfred R. Holzwarth

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.

Original languageEnglish
Pages (from-to)3625-3631
Number of pages7
JournalFEBS Letters
Volume582
Issue number25-26
DOIs
Publication statusPublished - 29 Oct 2008

Keywords

  • Aggregation
  • Arabidopsis mutant
  • Chlorophyll fluorescence
  • LHCII
  • Non-photochemical quenching
  • Violaxanthin
  • Zeaxanthin

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