Abstract
Time-resolved fluorescence on oligomers of the main light-harvesting complex from higher plants indicate that in vitro oligomerization leads to the formation of a weakly coupled inter-trimer chlorophyll-chlorophyll (Chl) exciton state which converts in tens of ps into a state which is spectrally broad and has a strongly far-red enhanced fluorescence spectrum. Both its lifetime and spectrum show striking similarity with a 400 ps fluorescence component appearing in intact leaves of Arabidopsis when non-photochemical quenching (NPQ) is induced. The fluorescence components with high far-red/red ratio are thus a characteristic marker for NPQ conditions in vivo. The far-red emitting state is shown to be an emissive Chl-Chl charge transfer state which plays a crucial part in the quenching.
Original language | English |
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Pages (from-to) | 3625-3631 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 582 |
Issue number | 25-26 |
DOIs | |
Publication status | Published - 29 Oct 2008 |
Keywords
- Aggregation
- Arabidopsis mutant
- Chlorophyll fluorescence
- LHCII
- Non-photochemical quenching
- Violaxanthin
- Zeaxanthin