Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Inland waters are large contributors to global carbon dioxide (CO2) emissions, in part due to the vulnerability of dissolved organic matter (DOM) to microbial decomposition and respiration to CO2 during transport through aquatic systems. To assess the degree of this vulnerability, aquatic DOM is often incubated in standardized biolability assays. These assays isolate the dissolved fraction of aquatic OM by size filtration prior to incubation. We test whether this size selection has an impact on the bacterial community composition and the consequent dynamics of DOM degradation using three different filtration strategies: 0.2 μm (filtered and inoculated), 0.7 μm (generally the most common DOM filter size) and 106 μm (unfiltered). We found that bacterial community composition, based on 16S rRNA amplicon sequencing, was significantly affected by the different filter sizes. At the same time, the filtration strategy also affected the DOM degradation dynamics, including the δ13C signature. However, the dynamics of these two responses were decoupled, suggesting that filtration primarily influences biolability assays through bacterial abundance and the presence of their associated predators. By the end of the 41-day incubations all treatments tended to converge on a common total DOM biolability level, with the 0.7 μm filtered incubations reaching this point the quickest. These results suggest that assays used to assess the total biolability of aquatic DOM should last long enough to remove filtration artefacts in the microbial population. Filtration strategy should also be taken into account when comparing results across biolability assays.

Original languageEnglish
Pages (from-to)7141-7154
Number of pages14
JournalBiogeosciences
Volume15
Issue number23
DOIs
Publication statusPublished - 30 Nov 2018

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dissolved organic matter
bacterial communities
community composition
artifact
assay
assays
incubation
carbon dioxide
degradation
vulnerability
filter
size selection
inland waters
breathing
respiration
ribosomal RNA
predator
decomposition
predators

Cite this

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title = "Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays",
abstract = "Inland waters are large contributors to global carbon dioxide (CO2) emissions, in part due to the vulnerability of dissolved organic matter (DOM) to microbial decomposition and respiration to CO2 during transport through aquatic systems. To assess the degree of this vulnerability, aquatic DOM is often incubated in standardized biolability assays. These assays isolate the dissolved fraction of aquatic OM by size filtration prior to incubation. We test whether this size selection has an impact on the bacterial community composition and the consequent dynamics of DOM degradation using three different filtration strategies: 0.2 μm (filtered and inoculated), 0.7 μm (generally the most common DOM filter size) and 106 μm (unfiltered). We found that bacterial community composition, based on 16S rRNA amplicon sequencing, was significantly affected by the different filter sizes. At the same time, the filtration strategy also affected the DOM degradation dynamics, including the δ13C signature. However, the dynamics of these two responses were decoupled, suggesting that filtration primarily influences biolability assays through bacterial abundance and the presence of their associated predators. By the end of the 41-day incubations all treatments tended to converge on a common total DOM biolability level, with the 0.7 μm filtered incubations reaching this point the quickest. These results suggest that assays used to assess the total biolability of aquatic DOM should last long enough to remove filtration artefacts in the microbial population. Filtration strategy should also be taken into account when comparing results across biolability assays.",
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Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays. / Dean, Joshua F.; Van Hal, Jurgen R.; Johannes Dolman, A.; Aerts, Rien; Weedon, James T.

In: Biogeosciences, Vol. 15, No. 23, 30.11.2018, p. 7141-7154.

Research output: Contribution to JournalArticleAcademicpeer-review

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