Flexibility in targeting and insertion during bacterial membrane protein biogenesis.

D.W. van Bloois, C.M. ten Hagen-Jongman, S. Luirink

Research output: Contribution to JournalArticleAcademicpeer-review


The biogenesis of Escherichia coli inner membrane proteins (IMPs) is assisted by targeting and insertion factors such as the signal recognition particle (SRP), the Sec-translocon and YidC with translocation of (large) periplasmic domains energized by SecA and the proton motive force (pmf). The use of these factors and forces is probably primarily determined by specific structural features of an IMP. To analyze these features we have engineered a set of model IMPs based on endogenous E. coli IMPs known to follow distinct targeting and insertion pathways. The modified model IMPs were analyzed for altered routing using an in vivo protease mapping approach. The data suggest a facultative use of different combinations of factors. © 2007 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)727-733
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Early online date20 Aug 2007
Publication statusPublished - 26 Oct 2007


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