TY - JOUR
T1 - Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry
AU - Ariese, F.
AU - Bader, A.N.
AU - Gooijer, C.
PY - 2008
Y1 - 2008
N2 - Fluorescence line-narrowing spectroscopy (FLNS) is a cryogenic technique that can be used to obtain high-resolution fluorescence spectra. The technique has mainly been used for fingerprint identification. However, this review focuses on the trend to use FLNS for probing purposes: obtaining information on interactions with the local environment or determining conformations. We briefly discuss the basics of FLNS, solute-matrix interactions and important instrumental aspects. We describe examples from various bioanalytical fields: DNA adducts of the aromatic carcinogen benzo[a]pyrene (BP); binding of BP metabolites to monoclonal antibodies or the estrogen receptor; FLNS studies of porphyrin-containing proteins; and, photosynthetic systems. As an illustration from the field of environmental chemistry, we discuss the use of luminescence line-narrowing spectroscopy - a special mode of FLNS - to study the complexation of lanthanides to humic substances. © 2008 Elsevier Ltd. All rights reserved.
AB - Fluorescence line-narrowing spectroscopy (FLNS) is a cryogenic technique that can be used to obtain high-resolution fluorescence spectra. The technique has mainly been used for fingerprint identification. However, this review focuses on the trend to use FLNS for probing purposes: obtaining information on interactions with the local environment or determining conformations. We briefly discuss the basics of FLNS, solute-matrix interactions and important instrumental aspects. We describe examples from various bioanalytical fields: DNA adducts of the aromatic carcinogen benzo[a]pyrene (BP); binding of BP metabolites to monoclonal antibodies or the estrogen receptor; FLNS studies of porphyrin-containing proteins; and, photosynthetic systems. As an illustration from the field of environmental chemistry, we discuss the use of luminescence line-narrowing spectroscopy - a special mode of FLNS - to study the complexation of lanthanides to humic substances. © 2008 Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.trac.2008.01.002
DO - 10.1016/j.trac.2008.01.002
M3 - Article
SN - 0165-9936
VL - 27
SP - 127
EP - 138
JO - TrAC. Trends in Analytical Chemistry
JF - TrAC. Trends in Analytical Chemistry
IS - 2
ER -