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Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

  • F. Ariese
  • , A.N. Bader
  • , C. Gooijer

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Fluorescence line-narrowing spectroscopy (FLNS) is a cryogenic technique that can be used to obtain high-resolution fluorescence spectra. The technique has mainly been used for fingerprint identification. However, this review focuses on the trend to use FLNS for probing purposes: obtaining information on interactions with the local environment or determining conformations. We briefly discuss the basics of FLNS, solute-matrix interactions and important instrumental aspects. We describe examples from various bioanalytical fields: DNA adducts of the aromatic carcinogen benzo[a]pyrene (BP); binding of BP metabolites to monoclonal antibodies or the estrogen receptor; FLNS studies of porphyrin-containing proteins; and, photosynthetic systems. As an illustration from the field of environmental chemistry, we discuss the use of luminescence line-narrowing spectroscopy - a special mode of FLNS - to study the complexation of lanthanides to humic substances. © 2008 Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)127-138
    JournalTrAC : Trends in Analytical Chemistry
    Volume27
    Issue number2
    DOIs
    Publication statusPublished - 2008

    UN SDGs

    This output contributes to the following UN Sustainable Development Goals (SDGs)

    1. SDG 6 - Clean Water and Sanitation
      SDG 6 Clean Water and Sanitation

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