Abstract
Amyloid oligomers are considered to be the relevant toxic species in many amyloid diseases and much research effort has been devoted to fully characterize these oligomers. Despite their importance, oligomers have proven to be difficult to characterize structurally. Information on their aggregation number is scarce, largely because standard techniques struggle to provide reliable results. In this chapter, we present two different methods that reproducibly yield fluorescently labeled α-Synuclein oligomers. We then discuss a new approach, combining single-molecule photobleaching and sub-stoichiometric fluorescent labeling, that we have developed to determine the aggregation number of supramolecular protein assemblies.
Original language | English |
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Pages (from-to) | 151-69 |
Number of pages | 19 |
Journal | Methods in Molecular Biology |
Volume | 1345 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- Fluorescent Dyes
- Humans
- Parkinson Disease
- Protein Multimerization
- Staining and Labeling
- alpha-Synuclein
- Journal Article
- Research Support, Non-U.S. Gov't