Formation of pyrene metabolites by the terrestrial isopod porcellio scaber.

G.J. Stroomberg, C. Reuther, I. Kozin, T.C. van Brummelen, C.A.M. van Gestel, C. Gooijer, W.P. Cofino

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The formation of pyrene metabolites by the terrestrial isopod Porcellio scaber (Latr.) was studied following exposure for 13 days to 100 μg/g pyrene in its food. An alkaline hydrolysis method with subsequent liquid-liquid extraction was developed to determine the metabolite content, especially 1-hydroxypyrene. Synchronous Fluorescence Spectroscopy (SFS) was explored as a fast screening method and HPLC was applied as a complementary method for confirmation of the SFS results. In Porcellio scaber the amount of 1-hydroxypyrene exceeded that of pyrene, which indicates that pyrene is being metabolized extensively. SFS and HPLC demonstrated that Porcellio scaber also forms a number of other fluorescent metabolites. These metabolites exhibit fluorescence at the same excitation wavelength as 1-hydroxypyrene, possibly indicating a similar fluorophore structure. Determination of 1-hydroxypyrene is considered as a first step towards an improved environmental risk assessment of PAH exposure, as pyrene is a predominant member of this class of environmental contaminants and 1-hydroxypyrene is its major metabolite.
Original languageEnglish
Pages (from-to)1905-1914
JournalChemosphere
Volume33
Issue number10
DOIs
Publication statusPublished - 1996

Fingerprint

Isopoda
Pyrene
isopod
Metabolites
pyrene
metabolite
fluorescence spectroscopy
Fluorescence Spectrometry
Fluorescence spectroscopy
High Pressure Liquid Chromatography
Liquid-Liquid Extraction
liquid
Fluorophores
Liquids
Polycyclic aromatic hydrocarbons
Risk assessment
hydrolysis
Hydrolysis
Screening
PAH

Cite this

Stroomberg, G. J., Reuther, C., Kozin, I., van Brummelen, T. C., van Gestel, C. A. M., Gooijer, C., & Cofino, W. P. (1996). Formation of pyrene metabolites by the terrestrial isopod porcellio scaber. Chemosphere, 33(10), 1905-1914. https://doi.org/10.1016/0045-6535(96)00316-5
Stroomberg, G.J. ; Reuther, C. ; Kozin, I. ; van Brummelen, T.C. ; van Gestel, C.A.M. ; Gooijer, C. ; Cofino, W.P. / Formation of pyrene metabolites by the terrestrial isopod porcellio scaber. In: Chemosphere. 1996 ; Vol. 33, No. 10. pp. 1905-1914.
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abstract = "The formation of pyrene metabolites by the terrestrial isopod Porcellio scaber (Latr.) was studied following exposure for 13 days to 100 μg/g pyrene in its food. An alkaline hydrolysis method with subsequent liquid-liquid extraction was developed to determine the metabolite content, especially 1-hydroxypyrene. Synchronous Fluorescence Spectroscopy (SFS) was explored as a fast screening method and HPLC was applied as a complementary method for confirmation of the SFS results. In Porcellio scaber the amount of 1-hydroxypyrene exceeded that of pyrene, which indicates that pyrene is being metabolized extensively. SFS and HPLC demonstrated that Porcellio scaber also forms a number of other fluorescent metabolites. These metabolites exhibit fluorescence at the same excitation wavelength as 1-hydroxypyrene, possibly indicating a similar fluorophore structure. Determination of 1-hydroxypyrene is considered as a first step towards an improved environmental risk assessment of PAH exposure, as pyrene is a predominant member of this class of environmental contaminants and 1-hydroxypyrene is its major metabolite.",
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Stroomberg, GJ, Reuther, C, Kozin, I, van Brummelen, TC, van Gestel, CAM, Gooijer, C & Cofino, WP 1996, 'Formation of pyrene metabolites by the terrestrial isopod porcellio scaber.' Chemosphere, vol. 33, no. 10, pp. 1905-1914. https://doi.org/10.1016/0045-6535(96)00316-5

Formation of pyrene metabolites by the terrestrial isopod porcellio scaber. / Stroomberg, G.J.; Reuther, C.; Kozin, I.; van Brummelen, T.C.; van Gestel, C.A.M.; Gooijer, C.; Cofino, W.P.

In: Chemosphere, Vol. 33, No. 10, 1996, p. 1905-1914.

Research output: Contribution to JournalArticleAcademicpeer-review

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AB - The formation of pyrene metabolites by the terrestrial isopod Porcellio scaber (Latr.) was studied following exposure for 13 days to 100 μg/g pyrene in its food. An alkaline hydrolysis method with subsequent liquid-liquid extraction was developed to determine the metabolite content, especially 1-hydroxypyrene. Synchronous Fluorescence Spectroscopy (SFS) was explored as a fast screening method and HPLC was applied as a complementary method for confirmation of the SFS results. In Porcellio scaber the amount of 1-hydroxypyrene exceeded that of pyrene, which indicates that pyrene is being metabolized extensively. SFS and HPLC demonstrated that Porcellio scaber also forms a number of other fluorescent metabolites. These metabolites exhibit fluorescence at the same excitation wavelength as 1-hydroxypyrene, possibly indicating a similar fluorophore structure. Determination of 1-hydroxypyrene is considered as a first step towards an improved environmental risk assessment of PAH exposure, as pyrene is a predominant member of this class of environmental contaminants and 1-hydroxypyrene is its major metabolite.

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