TY - JOUR
T1 - Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma
AU - Zandieh Doulabi, B.
PY - 2019/4/20
Y1 - 2019/4/20
N2 - Introdution: Cationic liposomes have been presented for gene delivery as an alternative vector instead of viral vectors. A major challenge associated with siRNA delivery is the instability of liposomes, which is still a serious problem. The aim of this study was to provide an appropriate formulation to overcome this instability.Methods: In the present study (Scientific-Fundamental, Experimental-Laboratory Study), liposomal formulation containing soy phosphatidylcholine, cationic DOTAP, cholesterol and polyethylene glycole was synthesized by thin-film hydration method and the siRNA were loaded on liposomes through incubation. In the following; the optimization of siRNA loading was on the agenda. Then the parameters related to size, zeta potential, polydispersity index and lon-term stability of siRNA-liposomes complex were reported. The Data were analyzed by GraphPad Prism version 7 Software. All data were repeated three times and reported as mean±standard deviation.Results: In this study we were able to produce siRNA lipoplex with high loading efficiency of siRNA. The produced nanoparticles did not agglomerate and were stable at 4 oC for 3 months. This nanosystem could successfully deliver siRNA to normal bone cells. Studies have shown that the blank system (no gene) had no toxicity.Conclusion: The prepared PEGylated liposomes have a great potential for delivery of siRNA to bone cells.
AB - Introdution: Cationic liposomes have been presented for gene delivery as an alternative vector instead of viral vectors. A major challenge associated with siRNA delivery is the instability of liposomes, which is still a serious problem. The aim of this study was to provide an appropriate formulation to overcome this instability.Methods: In the present study (Scientific-Fundamental, Experimental-Laboratory Study), liposomal formulation containing soy phosphatidylcholine, cationic DOTAP, cholesterol and polyethylene glycole was synthesized by thin-film hydration method and the siRNA were loaded on liposomes through incubation. In the following; the optimization of siRNA loading was on the agenda. Then the parameters related to size, zeta potential, polydispersity index and lon-term stability of siRNA-liposomes complex were reported. The Data were analyzed by GraphPad Prism version 7 Software. All data were repeated three times and reported as mean±standard deviation.Results: In this study we were able to produce siRNA lipoplex with high loading efficiency of siRNA. The produced nanoparticles did not agglomerate and were stable at 4 oC for 3 months. This nanosystem could successfully deliver siRNA to normal bone cells. Studies have shown that the blank system (no gene) had no toxicity.Conclusion: The prepared PEGylated liposomes have a great potential for delivery of siRNA to bone cells.
UR - http://dx.doi.org/10.18502/ssu.v26i12.660
U2 - 10.18502/ssu.v26i12.660
DO - 10.18502/ssu.v26i12.660
M3 - Article
SN - 2228-5733
JO - Journal of Shahid Sadoughi University of Medical Sciences
JF - Journal of Shahid Sadoughi University of Medical Sciences
ER -