Forward-scattering degenerate four-wave mixing as a potential laser-based absorption detection method in liquid separation systems: coupling to conventional-size liquid chromatography.

T. de Beer, G.Ph. Hoornweg, G.J. Grootendorst, N.H. Velthorst, C. Gooijer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

An explorative study on the compatibility of liquid separation systems, such as (micro) liquid chromatography (LC) and capillary electrophoresis (CE), and forward-scattering degenerate four-wave mixing (F-D4WM) as a detection method is presented. F-D4WM is a laser-based technique showing some analogy with holographic spectroscopy: a signal on a theoretical dark background is observed as a result of light absorption by an analyte. Parameters considered are solvent composition (focussing on acetonitrile, methanol and water; mobile phases in LC and CE), detector cell construction, and influences of laser beam powers. A specially designed detector cell has been developed to meet the Brewster condition, both at the air-quartz and the quartz-liquid boundaries. For practical reasons, the tested cell has an optical pathlength of 1 mm; reduction to 100 μm is required to apply the cell in microseparations. The F-D4WM technique has been involved for detection in a conventional-size, reversed-phase LC separation of 1- and 2-aminoanthraquinones. The detection limit obtained (for the 1 mm cell) is 2 x 10
Original languageEnglish
Pages (from-to)189-197
JournalAnalytica Chimica Acta
Volume330
DOIs
Publication statusPublished - 1996

Fingerprint Dive into the research topics of 'Forward-scattering degenerate four-wave mixing as a potential laser-based absorption detection method in liquid separation systems: coupling to conventional-size liquid chromatography.'. Together they form a unique fingerprint.

Cite this