Fourier conjugate adaptive optics for deep-tissue large field of view imaging

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of adaptive optics (AO), which are aimed at pre-compensation of these distortions, still suffer from a limited field of view and imaging depth as well as inconvenient microscope design. Here, I propose and investigate a new approach to overcome these limitations: Fourier image plane conjugate AO. Two experimental designs of the new approach are carefully studied, and an accurate comparison between different methods of AO is presented. Fourier conjugate AO provides a larger field of view, which can only be limited by the angular memory effect, and allows the optimal use of the spatial light modulator. Moreover, theoretically possible imaging depth of Fourier conjugate AO is limited only by the working distance of the objective and not by the microscope design.

Original languageEnglish
Pages (from-to)9803-9808
Number of pages6
JournalApplied Optics
Volume57
Issue number33
DOIs
Publication statusPublished - 20 Nov 2018

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Adaptive optics
adaptive optics
field of view
Tissue
Imaging techniques
Microscopes
microscopes
light modulators
Aberrations
Design of experiments
Optical microscopy
aberration
optics
microscopy
Data storage equipment

Cite this

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title = "Fourier conjugate adaptive optics for deep-tissue large field of view imaging",
abstract = "Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of adaptive optics (AO), which are aimed at pre-compensation of these distortions, still suffer from a limited field of view and imaging depth as well as inconvenient microscope design. Here, I propose and investigate a new approach to overcome these limitations: Fourier image plane conjugate AO. Two experimental designs of the new approach are carefully studied, and an accurate comparison between different methods of AO is presented. Fourier conjugate AO provides a larger field of view, which can only be limited by the angular memory effect, and allows the optimal use of the spatial light modulator. Moreover, theoretically possible imaging depth of Fourier conjugate AO is limited only by the working distance of the objective and not by the microscope design.",
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Fourier conjugate adaptive optics for deep-tissue large field of view imaging. / Amitonova, Lyubov V.

In: Applied Optics, Vol. 57, No. 33, 20.11.2018, p. 9803-9808.

Research output: Contribution to JournalArticleAcademicpeer-review

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