Functional electric field changes in photoactivated proteins revealed by ultrafast Stark spectroscopy of the Trp residues

J. Leonard, E. Portuondo-Campa, A. Cannizzo, F. van Mourik, G. van der Zwan, J. Tittor, S. Haacke, M. Chergui

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Ultrafast transient absorption spectroscopy of wild-type bacteriorhodopsin (WT bR) and 2 tryptophan mutants (W86F and W182F) is performed with visible light excitation (pump) and UV probe. The aim is to investigate the photoinduced change in the charge distribution with 50-fs time resolution by probing the effects on the tryptophan absorption bands. A systematic, quantitative comparison of the transient absorption of the 3 samples is carried out. The main result is the absence in the W86F mutant of a transient induced absorption band observed at ≈300-310 nm in WT bR and W182F. A simple model describing the dipolar interaction of the retinal moiety with the 2 tryptophan residues of interest allows us to reproduce the dominant features of the transient signals observed in the 3 samples at ultrashort pump-probe delays. In particular, we show that Trp
Original languageEnglish
Pages (from-to)7718-23
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number19
DOIs
Publication statusPublished - 2009

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