Functionally different α-synuclein inclusions yield insight into Parkinson's disease pathology

Christian C Raiss, Theresa S Braun, Irene B M Konings, Heinrich Grabmayr, Gerco C Hassink, Arshdeep Sidhu, Joost le Feber, Andreas R Bausch, Casper Jansen, Vinod Subramaniam, Mireille M A E Claessens

    Research output: Contribution to JournalArticle

    Abstract

    The formation of α-synuclein (α-S) amyloid aggregates, called Lewy bodies (LBs), is a hallmark of Parkinson's disease (PD). The function of LBs in the disease process is however still unclear; they have been associated with both neuroprotection and toxicity. To obtain insight into this contradiction, we induced the formation of α-S inclusions, using three different induction methods in SH-SY5Y cells and rat-derived primary neuronal cells. Using confocal and STED microscopy we observed induction-dependent differences in α-S inclusion morphology, location and function. The aggregation of α-S in functionally different compartments correlates with the toxicity of the induction method measured in viability assays. The most cytotoxic treatment largely correlates with the formation of proteasome-associated, juxta-nuclear inclusions. With less toxic methods cytosolic deposits that are not associated with the proteasome are more prevalent. The distribution of α-S over at least two different types of inclusions is not limited to cell models, but is also observed in primary neuronal cells and in human mesencephalon. The existence of functionally different LBs, in vivo and in vitro, gives important insights in the impact of Lewy Body formation on neuronal functioning and may thereby provide a platform for discovering therapeutics.

    Original languageEnglish
    Pages (from-to)23116
    JournalScientific Reports
    Volume6
    DOIs
    Publication statusPublished - 17 Mar 2016

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    Keywords

    • Amyloid
    • Animals
    • Cells, Cultured
    • Green Fluorescent Proteins
    • Humans
    • Lewy Bodies
    • Mesencephalon
    • Microscopy, Atomic Force
    • Microscopy, Confocal
    • Neurons
    • Parkinson Disease
    • Proteasome Endopeptidase Complex
    • Protein Aggregates
    • Rats
    • Rats, Wistar
    • Recombinant Fusion Proteins
    • Transfection
    • alpha-Synuclein
    • Journal Article
    • Research Support, Non-U.S. Gov't

    Cite this

    Raiss, C. C., Braun, T. S., Konings, I. B. M., Grabmayr, H., Hassink, G. C., Sidhu, A., ... Claessens, M. M. A. E. (2016). Functionally different α-synuclein inclusions yield insight into Parkinson's disease pathology. Scientific Reports, 6, 23116. https://doi.org/10.1038/srep23116