Abstract
Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.
| Original language | English |
|---|---|
| Article number | 1803 |
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | Frontiers in Microbiology |
| Volume | 9 |
| Issue number | AUGUST |
| DOIs | |
| Publication status | Published - 3 Aug 2018 |
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Keywords
- Galactose transporter
- GalP
- Lactococcus lactis
- PEP-transferase system
- PTS
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Further elucidation of galactose utilization in Lactococcus lactis MG1363. / Solopova, Ana; Bachmann, Herwig; Teusink, Bas; Kok, Jan; Kuipers, Oscar P.
In: Frontiers in Microbiology, Vol. 9, No. AUGUST, 1803, 03.08.2018, p. 1-9.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Further elucidation of galactose utilization in Lactococcus lactis MG1363
AU - Solopova, Ana
AU - Bachmann, Herwig
AU - Teusink, Bas
AU - Kok, Jan
AU - Kuipers, Oscar P.
PY - 2018/8/3
Y1 - 2018/8/3
N2 - Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.
AB - Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.
KW - Galactose transporter
KW - GalP
KW - Lactococcus lactis
KW - PEP-transferase system
KW - PTS
UR - http://www.scopus.com/inward/record.url?scp=85051055113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051055113&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2018.01803
DO - 10.3389/fmicb.2018.01803
M3 - Article
VL - 9
SP - 1
EP - 9
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - AUGUST
M1 - 1803
ER -