Further elucidation of galactose utilization in Lactococcus lactis MG1363

Ana Solopova, Herwig Bachmann, Bas Teusink, Jan Kok, Oscar P. Kuipers

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.

Original languageEnglish
Article number1803
Pages (from-to)1-9
Number of pages9
JournalFrontiers in Microbiology
Volume9
Issue numberAUGUST
DOIs
Publication statusPublished - 3 Aug 2018

Fingerprint

Lactococcus lactis
Galactose
Lactose
Lactococcus
Membrane Transport Proteins
Gene Expression Profiling
Mannose
Plasmids
Adenosine Triphosphate
Glucose

Keywords

  • Galactose transporter
  • GalP
  • Lactococcus lactis
  • PEP-transferase system
  • PTS

Cite this

Solopova, Ana ; Bachmann, Herwig ; Teusink, Bas ; Kok, Jan ; Kuipers, Oscar P. / Further elucidation of galactose utilization in Lactococcus lactis MG1363. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. AUGUST. pp. 1-9.
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Further elucidation of galactose utilization in Lactococcus lactis MG1363. / Solopova, Ana; Bachmann, Herwig; Teusink, Bas; Kok, Jan; Kuipers, Oscar P.

In: Frontiers in Microbiology, Vol. 9, No. AUGUST, 1803, 03.08.2018, p. 1-9.

Research output: Contribution to JournalArticleAcademicpeer-review

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