Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine

N P Vermeulen, D. De Roode, D D Breimer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.

Original languageEnglish
Pages (from-to)333-42
Number of pages10
JournalJournal of Chromatography A
Volume137
Issue number2
DOIs
Publication statusPublished - 21 Jul 1977

Fingerprint

Pemoline
Gases
Urine
Diazomethane
Pharmacokinetics
Hydrochloric Acid
Liquid chromatography
Gas chromatography
Assays
Screening
Nitrogen
Doping (additives)
Gas Chromatography
Detectors
Oral Administration
Pharmaceutical Preparations
Injections

Keywords

  • Chromatography, Gas
  • Humans
  • Hydrolysis
  • Mass Screening
  • Methods
  • Microchemistry
  • Oxazoles
  • Pemoline
  • Journal Article

Cite this

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title = "Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine",
abstract = "A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.",
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year = "1977",
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language = "English",
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Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine. / Vermeulen, N P; De Roode, D.; Breimer, D D.

In: Journal of Chromatography A, Vol. 137, No. 2, 21.07.1977, p. 333-42.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine

AU - Vermeulen, N P

AU - De Roode, D.

AU - Breimer, D D

PY - 1977/7/21

Y1 - 1977/7/21

N2 - A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.

AB - A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.

KW - Chromatography, Gas

KW - Humans

KW - Hydrolysis

KW - Mass Screening

KW - Methods

KW - Microchemistry

KW - Oxazoles

KW - Pemoline

KW - Journal Article

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DO - 10.1016/S0021-9673(00)81356-4

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