Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine

N P Vermeulen; D. De Roode; D D Breimer

doi:10.1016/S0021-9673(00)81356-4

Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine

N P Vermeulen, D. De Roode, D D Breimer

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.

Original language	English
Pages (from-to)	333-42
Number of pages	10
Journal	Journal of Chromatography A
Volume	137
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(00)81356-4
Publication status	Published - 21 Jul 1977

Keywords

Chromatography, Gas
Humans
Hydrolysis
Mass Screening
Methods
Microchemistry
Oxazoles
Pemoline
Journal Article

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title = "Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine",

abstract = "A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.",

keywords = "Chromatography, Gas, Humans, Hydrolysis, Mass Screening, Methods, Microchemistry, Oxazoles, Pemoline, Journal Article",

author = "Vermeulen, {N P} and {De Roode}, D. and Breimer, {D D}",

year = "1977",

month = jul,

day = "21",

doi = "10.1016/S0021-9673(00)81356-4",

language = "English",

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pages = "333--42",

journal = "Journal of chromatography A",

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T1 - Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine

AU - Vermeulen, N P

AU - De Roode, D.

AU - Breimer, D D

PY - 1977/7/21

Y1 - 1977/7/21

N2 - A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.

AB - A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.

KW - Chromatography, Gas

KW - Humans

KW - Hydrolysis

KW - Mass Screening

KW - Methods

KW - Microchemistry

KW - Oxazoles

KW - Pemoline

KW - Journal Article

U2 - 10.1016/S0021-9673(00)81356-4

DO - 10.1016/S0021-9673(00)81356-4

M3 - Article

C2 - 881457

VL - 137

SP - 333

EP - 342

JO - Journal of chromatography A

JF - Journal of chromatography A

SN - 0021-9673

IS - 2

ER -

Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine

Abstract

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