Abstract
A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.
| Original language | English |
|---|---|
| Pages (from-to) | 333-42 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 137 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 21 Jul 1977 |
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Keywords
- Chromatography, Gas
- Humans
- Hydrolysis
- Mass Screening
- Methods
- Microchemistry
- Oxazoles
- Pemoline
- Journal Article
Cite this
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Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine. / Vermeulen, N P; De Roode, D.; Breimer, D D.
In: Journal of Chromatography A, Vol. 137, No. 2, 21.07.1977, p. 333-42.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Gas chromatographic determination of pemoline as 5-phenyl-2,4-oxazolidinedione in human urine
AU - Vermeulen, N P
AU - De Roode, D.
AU - Breimer, D D
PY - 1977/7/21
Y1 - 1977/7/21
N2 - A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.
AB - A simple gas chromatographic assay of the psychostimulant pemoline in human urine has been developed. Instead of extraction of the drug from urine, it is hydrolysed to 5-phenyl-2,4-oxazolidinedione with 1 N hydrochloric acid. After the extraction, this compound is methylated with diazomethane and determined by gas-liquid chromatography using a nitrogen-selective detector and a solid injection system. The method has been applied in preliminary human pharmacokinetic studies, by measuring the urinary excretion rate of pemoline following oral administration. At present, the screening procedures for doping control do not involve the detection of pemoline, but the method described can easily be incorporated in such procedures.
KW - Chromatography, Gas
KW - Humans
KW - Hydrolysis
KW - Mass Screening
KW - Methods
KW - Microchemistry
KW - Oxazoles
KW - Pemoline
KW - Journal Article
U2 - 10.1016/S0021-9673(00)81356-4
DO - 10.1016/S0021-9673(00)81356-4
M3 - Article
VL - 137
SP - 333
EP - 342
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -