TY - JOUR
T1 - Generic GPCR residue numbers - Aligning topology maps while minding the gaps
AU - Isberg, Vignir
AU - de Graaf, C.
AU - Bortolato, Andrea
AU - Cherezov, Vadim
AU - Katritch, Vsevolod
AU - Marshall, Fiona H.
AU - Mordalski, Stefan
AU - Pin, Jean Philippe
AU - Stevens, Raymond C.
AU - Vriend, Gerrit
AU - Gloriam, David E.
PY - 2015
Y1 - 2015
N2 - Generic residue numbers facilitate comparisons of, for example, mutational effects, ligand interactions, and structural motifs. The numbering scheme by Ballesteros and Weinstein for residues within the class A GPCRs (G protein-coupled receptors) has more than 1100 citations, and the recent crystal structures for classes B, C, and F now call for a community consensus in residue numbering within and across these classes. Furthermore, the structural era has uncovered helix bulges and constrictions that offset the generic residue numbers. The use of generic residue numbers depends on convenient access by pharmacologists, chemists, and structural biologists. We review the generic residue numbering schemes for each GPCR class, as well as a complementary structure-based scheme, and provide illustrative examples and GPCR database (GPCRDB) web tools to number any receptor sequence or structure.
AB - Generic residue numbers facilitate comparisons of, for example, mutational effects, ligand interactions, and structural motifs. The numbering scheme by Ballesteros and Weinstein for residues within the class A GPCRs (G protein-coupled receptors) has more than 1100 citations, and the recent crystal structures for classes B, C, and F now call for a community consensus in residue numbering within and across these classes. Furthermore, the structural era has uncovered helix bulges and constrictions that offset the generic residue numbers. The use of generic residue numbers depends on convenient access by pharmacologists, chemists, and structural biologists. We review the generic residue numbering schemes for each GPCR class, as well as a complementary structure-based scheme, and provide illustrative examples and GPCR database (GPCRDB) web tools to number any receptor sequence or structure.
KW - G protein-coupled receptor
KW - ligand binding
KW - mutational effects
KW - sequence alignments
KW - structural motifs
UR - http://www.scopus.com/inward/record.url?scp=84920166693&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920166693&partnerID=8YFLogxK
U2 - 10.1016/j.tips.2014.11.001
DO - 10.1016/j.tips.2014.11.001
M3 - Review article
C2 - 25541108
AN - SCOPUS:84920166693
SN - 0165-6147
VL - 36
SP - 22
EP - 31
JO - Trends in Pharmacological Sciences
JF - Trends in Pharmacological Sciences
IS - 1
ER -