Abstract
Antibiotic resistance necessitates the search for new bioactive compounds with novel mechanisms of action. Natural products derived from bacteria and fungi are widely used in the field of medicine and new environments can be explored as sources of antimicrobials. Bacteria associated with springtails have shown high inhibitory activity against pathogens. Here, we characterized a bacterial strain with high potential for antimicrobial activity, isolated from the gut of the springtail Folsomia candida Willem (Collembola: Isotomidae). The strain was characterized using the ‘analytical profile index’ and the ‘minimal inhibitory concentration’ assay to test for antibiotic resistance. Agar overlay and agar disk diffusion assays were used to test the inhibitory activity of the strain and its extract against a variety of pathogens, and reporter assays were used to investigate the mode of action. High-performance liquid chromatography was used to analyze and fractionate the extract of bacterial culture, followed by additional assays on the fractions. The genome of the strain was screened for presence of antibiotic resistance genes and secondary metabolite gene clusters. The isolate was identified as Bacillus toyonensis Jiménez et al., but it displayed differences in metabolic profile when compared to the type species. The isolate was highly resistant to penicillin and inhibited the growth of a variety of pathogenic microorganisms. Genome analysis revealed an enrichment of resistance genes for β-lactam antibiotics compared to the type isolate. Also, secondary metabolite clusters involved in the production of siderophores, bacteriocins, and nonribosomal peptide synthetases were identified. In conclusion, a unique Bacillus strain was isolated from the gut of F. candida, for which we provide evidence of inhibitory activity against an array of pathogens. This, coupled with high resistance to penicillin as substantiated by the presence of resistance genes, points to the potential of B. toyonensis VU-DES13 to provide a new source of antimicrobial compounds.
| Original language | English |
|---|---|
| Pages (from-to) | 269-285 |
| Number of pages | 17 |
| Journal | Entomologia experimentalis et applicata |
| Volume | 167 |
| Issue number | 3 |
| Early online date | 4 Mar 2019 |
| DOIs | |
| Publication status | Published - Mar 2019 |
Fingerprint
Bibliographical note
Special Issue: Symbionts in Insect Biology and Pest ControlKeywords
- antibiotic resistance
- Collembola
- colonization resistance
- inhibition
- Isotomidae
- pathogens
- springtail
Cite this
}
Genome annotation and antimicrobial properties of Bacillus toyonensis VU-DES13, isolated from the Folsomia candida gut. / Agamennone, Valeria; van Straalen, Joeri; Brouwer, Abraham; de Boer, Tjalf E.; Hensbergen, Paul J.; Zaagman, Niels; Braster, Martin; van Straalen, Nico M.; Roelofs, Dick; Janssens, Thierry K.S.
In: Entomologia experimentalis et applicata, Vol. 167, No. 3, 03.2019, p. 269-285.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Genome annotation and antimicrobial properties of Bacillus toyonensis VU-DES13, isolated from the Folsomia candida gut
AU - Agamennone, Valeria
AU - van Straalen, Joeri
AU - Brouwer, Abraham
AU - de Boer, Tjalf E.
AU - Hensbergen, Paul J.
AU - Zaagman, Niels
AU - Braster, Martin
AU - van Straalen, Nico M.
AU - Roelofs, Dick
AU - Janssens, Thierry K.S.
N1 - Special Issue: Symbionts in Insect Biology and Pest Control
PY - 2019/3
Y1 - 2019/3
N2 - Antibiotic resistance necessitates the search for new bioactive compounds with novel mechanisms of action. Natural products derived from bacteria and fungi are widely used in the field of medicine and new environments can be explored as sources of antimicrobials. Bacteria associated with springtails have shown high inhibitory activity against pathogens. Here, we characterized a bacterial strain with high potential for antimicrobial activity, isolated from the gut of the springtail Folsomia candida Willem (Collembola: Isotomidae). The strain was characterized using the ‘analytical profile index’ and the ‘minimal inhibitory concentration’ assay to test for antibiotic resistance. Agar overlay and agar disk diffusion assays were used to test the inhibitory activity of the strain and its extract against a variety of pathogens, and reporter assays were used to investigate the mode of action. High-performance liquid chromatography was used to analyze and fractionate the extract of bacterial culture, followed by additional assays on the fractions. The genome of the strain was screened for presence of antibiotic resistance genes and secondary metabolite gene clusters. The isolate was identified as Bacillus toyonensis Jiménez et al., but it displayed differences in metabolic profile when compared to the type species. The isolate was highly resistant to penicillin and inhibited the growth of a variety of pathogenic microorganisms. Genome analysis revealed an enrichment of resistance genes for β-lactam antibiotics compared to the type isolate. Also, secondary metabolite clusters involved in the production of siderophores, bacteriocins, and nonribosomal peptide synthetases were identified. In conclusion, a unique Bacillus strain was isolated from the gut of F. candida, for which we provide evidence of inhibitory activity against an array of pathogens. This, coupled with high resistance to penicillin as substantiated by the presence of resistance genes, points to the potential of B. toyonensis VU-DES13 to provide a new source of antimicrobial compounds.
AB - Antibiotic resistance necessitates the search for new bioactive compounds with novel mechanisms of action. Natural products derived from bacteria and fungi are widely used in the field of medicine and new environments can be explored as sources of antimicrobials. Bacteria associated with springtails have shown high inhibitory activity against pathogens. Here, we characterized a bacterial strain with high potential for antimicrobial activity, isolated from the gut of the springtail Folsomia candida Willem (Collembola: Isotomidae). The strain was characterized using the ‘analytical profile index’ and the ‘minimal inhibitory concentration’ assay to test for antibiotic resistance. Agar overlay and agar disk diffusion assays were used to test the inhibitory activity of the strain and its extract against a variety of pathogens, and reporter assays were used to investigate the mode of action. High-performance liquid chromatography was used to analyze and fractionate the extract of bacterial culture, followed by additional assays on the fractions. The genome of the strain was screened for presence of antibiotic resistance genes and secondary metabolite gene clusters. The isolate was identified as Bacillus toyonensis Jiménez et al., but it displayed differences in metabolic profile when compared to the type species. The isolate was highly resistant to penicillin and inhibited the growth of a variety of pathogenic microorganisms. Genome analysis revealed an enrichment of resistance genes for β-lactam antibiotics compared to the type isolate. Also, secondary metabolite clusters involved in the production of siderophores, bacteriocins, and nonribosomal peptide synthetases were identified. In conclusion, a unique Bacillus strain was isolated from the gut of F. candida, for which we provide evidence of inhibitory activity against an array of pathogens. This, coupled with high resistance to penicillin as substantiated by the presence of resistance genes, points to the potential of B. toyonensis VU-DES13 to provide a new source of antimicrobial compounds.
KW - antibiotic resistance
KW - Collembola
KW - colonization resistance
KW - inhibition
KW - Isotomidae
KW - pathogens
KW - springtail
UR - http://www.scopus.com/inward/record.url?scp=85062595907&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062595907&partnerID=8YFLogxK
U2 - 10.1111/eea.12763
DO - 10.1111/eea.12763
M3 - Article
VL - 167
SP - 269
EP - 285
JO - Entomologia experimentalis et applicata
JF - Entomologia experimentalis et applicata
SN - 0013-8703
IS - 3
ER -