Geobacteraceae community composition is related to hydrochemistry and biodegradation in an iron-reducing aquifer polluted by a neighboring landfill.

B. Lin, M. Braster, B.M. van Breukelen, H.W. van Verseveld, H.V. Westerhoff, W.F.M. Roling

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Relationships between community composition of the iron-reducing Geobacteraceae, pollution levels, and the occurrence of biodegradation were established for an iron-reducing aquifer polluted with landfill leachate by using cultivation-independent Geobacteraceae 16S rRNA gene-targeting techniques. Numerical analysis of denaturing gradient gel electrophoresis (DGGE) profiles and sequencing revealed a high Geobacteraceae diversity and showed that community composition within the leachate plume differed considerably from that of the unpolluted aquifer. This suggests that pollution has selected for specific species out of a large pool of Geobacteraceae. DGGE profiles of polluted groundwater taken near the landfill (6- to 39-m distance) clustered together. DGGE profiles from less-polluted groundwater taken further downstream did not fall in the same cluster. Several individual DGGE bands were indicative of either the redox process or the level of pollution. This included a pollution-indicative band that dominated the DGGE profiles from groundwater samples taken close to the landfill (6 to 39 m distance). The clustering of these profiles and the dominance by a single DGGE band corresponded to the part of the aquifer where organic micropollutants and reactive dissolved organic matter were attenuated at relatively high rates. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)5983-5991
Number of pages9
JournalApplied and Environmental Microbiology
Volume71
DOIs
Publication statusPublished - 2005

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Geobacteraceae
Waste Disposal Facilities
Denaturing Gradient Gel Electrophoresis
hydrochemistry
Groundwater
landfills
denaturing gradient gel electrophoresis
biodegradation
aquifers
community composition
landfill
electrokinesis
Iron
gel
aquifer
iron
pollution
groundwater
Chemical Water Pollutants
landfill leachates

Cite this

@article{60571068f0a14b23873ba1bfa4c5be5f,
title = "Geobacteraceae community composition is related to hydrochemistry and biodegradation in an iron-reducing aquifer polluted by a neighboring landfill.",
abstract = "Relationships between community composition of the iron-reducing Geobacteraceae, pollution levels, and the occurrence of biodegradation were established for an iron-reducing aquifer polluted with landfill leachate by using cultivation-independent Geobacteraceae 16S rRNA gene-targeting techniques. Numerical analysis of denaturing gradient gel electrophoresis (DGGE) profiles and sequencing revealed a high Geobacteraceae diversity and showed that community composition within the leachate plume differed considerably from that of the unpolluted aquifer. This suggests that pollution has selected for specific species out of a large pool of Geobacteraceae. DGGE profiles of polluted groundwater taken near the landfill (6- to 39-m distance) clustered together. DGGE profiles from less-polluted groundwater taken further downstream did not fall in the same cluster. Several individual DGGE bands were indicative of either the redox process or the level of pollution. This included a pollution-indicative band that dominated the DGGE profiles from groundwater samples taken close to the landfill (6 to 39 m distance). The clustering of these profiles and the dominance by a single DGGE band corresponded to the part of the aquifer where organic micropollutants and reactive dissolved organic matter were attenuated at relatively high rates. Copyright {\circledC} 2005, American Society for Microbiology. All Rights Reserved.",
author = "B. Lin and M. Braster and {van Breukelen}, B.M. and {van Verseveld}, H.W. and H.V. Westerhoff and W.F.M. Roling",
year = "2005",
doi = "10.1128/AEM.71.10.5983-5991.2005",
language = "English",
volume = "71",
pages = "5983--5991",
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Geobacteraceae community composition is related to hydrochemistry and biodegradation in an iron-reducing aquifer polluted by a neighboring landfill. / Lin, B.; Braster, M.; van Breukelen, B.M.; van Verseveld, H.W.; Westerhoff, H.V.; Roling, W.F.M.

In: Applied and Environmental Microbiology, Vol. 71, 2005, p. 5983-5991.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Geobacteraceae community composition is related to hydrochemistry and biodegradation in an iron-reducing aquifer polluted by a neighboring landfill.

AU - Lin, B.

AU - Braster, M.

AU - van Breukelen, B.M.

AU - van Verseveld, H.W.

AU - Westerhoff, H.V.

AU - Roling, W.F.M.

PY - 2005

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AB - Relationships between community composition of the iron-reducing Geobacteraceae, pollution levels, and the occurrence of biodegradation were established for an iron-reducing aquifer polluted with landfill leachate by using cultivation-independent Geobacteraceae 16S rRNA gene-targeting techniques. Numerical analysis of denaturing gradient gel electrophoresis (DGGE) profiles and sequencing revealed a high Geobacteraceae diversity and showed that community composition within the leachate plume differed considerably from that of the unpolluted aquifer. This suggests that pollution has selected for specific species out of a large pool of Geobacteraceae. DGGE profiles of polluted groundwater taken near the landfill (6- to 39-m distance) clustered together. DGGE profiles from less-polluted groundwater taken further downstream did not fall in the same cluster. Several individual DGGE bands were indicative of either the redox process or the level of pollution. This included a pollution-indicative band that dominated the DGGE profiles from groundwater samples taken close to the landfill (6 to 39 m distance). The clustering of these profiles and the dominance by a single DGGE band corresponded to the part of the aquifer where organic micropollutants and reactive dissolved organic matter were attenuated at relatively high rates. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

U2 - 10.1128/AEM.71.10.5983-5991.2005

DO - 10.1128/AEM.71.10.5983-5991.2005

M3 - Article

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