Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN.

M.P. Bergman; A.J. Engering; HH Smits; SJ van Vliet; A.A. van Bodegraven; HP Wirth; ML Kapsenberg; C.M.J.E. Vandenbroucke-Grauls; Y. van Kooijk; B.J. Appelmelk

doi:10.1084/jem.20041061

Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN.

M.P. Bergman, A.J. Engering, HH Smits, SJ van Vliet, A.A. van Bodegraven, HP Wirth, ML Kapsenberg, C.M.J.E. Vandenbroucke-Grauls, Y. van Kooijk, B.J. Appelmelk

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Abstract

The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. pylori variants are able to bind to the C-type lectin DC-SIGN and present on gastric dendritic cells (DCs), and demonstrate that this interaction blocks T helper cell (Th)1 development. In contrast, Le- variants escape binding to DCs and induce a strong Th1 cell response. In addition, in gastric biopsies challenged ex vivo with Le+ variants that bind DC-SIGN, interleukin 6 production is decreased, indicative of increased immune suppression. Our data indicate a role for LPS phase variation and Le antigen expression by H. pylori in suppressing immune responses through DC-SIGN.

Original language	English
Pages (from-to)	979-90
Journal	Journal of Experimental Medicine
Volume	200
Issue number	8
DOIs	https://doi.org/10.1084/jem.20041061
Publication status	Published - 2004
Externally published	Yes

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Bergman, M. P., Engering, A. J., Smits, HH., van Vliet, SJ., van Bodegraven, A. A., Wirth, HP., Kapsenberg, ML., Vandenbroucke-Grauls, C. M. J. E., van Kooijk, Y., & Appelmelk, B. J. (2004). Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN. Journal of Experimental Medicine, 200(8), 979-90. https://doi.org/10.1084/jem.20041061

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title = "Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN.",

abstract = "The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. pylori variants are able to bind to the C-type lectin DC-SIGN and present on gastric dendritic cells (DCs), and demonstrate that this interaction blocks T helper cell (Th)1 development. In contrast, Le- variants escape binding to DCs and induce a strong Th1 cell response. In addition, in gastric biopsies challenged ex vivo with Le+ variants that bind DC-SIGN, interleukin 6 production is decreased, indicative of increased immune suppression. Our data indicate a role for LPS phase variation and Le antigen expression by H. pylori in suppressing immune responses through DC-SIGN.",

author = "M.P. Bergman and A.J. Engering and HH Smits and \{van Vliet\}, SJ and \{van Bodegraven\}, A.A. and HP Wirth and ML Kapsenberg and C.M.J.E. Vandenbroucke-Grauls and \{van Kooijk\}, Y. and B.J. Appelmelk",

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doi = "10.1084/jem.20041061",

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Bergman, MP, Engering, AJ, Smits, HH, van Vliet, SJ, van Bodegraven, AA, Wirth, HP, Kapsenberg, ML, Vandenbroucke-Grauls, CMJE, van Kooijk, Y & Appelmelk, BJ 2004, 'Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN.', Journal of Experimental Medicine, vol. 200, no. 8, pp. 979-90. https://doi.org/10.1084/jem.20041061

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T1 - Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN.

AU - Bergman, M.P.

AU - Engering, A.J.

AU - Smits, HH

AU - van Vliet, SJ

AU - van Bodegraven, A.A.

AU - Wirth, HP

AU - Kapsenberg, ML

AU - Vandenbroucke-Grauls, C.M.J.E.

AU - van Kooijk, Y.

AU - Appelmelk, B.J.

PY - 2004

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N2 - The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. pylori variants are able to bind to the C-type lectin DC-SIGN and present on gastric dendritic cells (DCs), and demonstrate that this interaction blocks T helper cell (Th)1 development. In contrast, Le- variants escape binding to DCs and induce a strong Th1 cell response. In addition, in gastric biopsies challenged ex vivo with Le+ variants that bind DC-SIGN, interleukin 6 production is decreased, indicative of increased immune suppression. Our data indicate a role for LPS phase variation and Le antigen expression by H. pylori in suppressing immune responses through DC-SIGN.

AB - The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. pylori variants are able to bind to the C-type lectin DC-SIGN and present on gastric dendritic cells (DCs), and demonstrate that this interaction blocks T helper cell (Th)1 development. In contrast, Le- variants escape binding to DCs and induce a strong Th1 cell response. In addition, in gastric biopsies challenged ex vivo with Le+ variants that bind DC-SIGN, interleukin 6 production is decreased, indicative of increased immune suppression. Our data indicate a role for LPS phase variation and Le antigen expression by H. pylori in suppressing immune responses through DC-SIGN.

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Helicobacter pylori modulates the T helper cell 1/T helper cell 2 balance through phase-variable interaction between lipopolysaccharide and DC-SIGN.

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