High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.

J. Kool, F. Heus, G.E. de Kloe, H. Lingeman, A.B. Smit, R. Leurs, I.J.P. de Esch, H. Irth, W.M.A. Niessen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

This study describes the evaluation, validation, and use of contactless postcolumn fractionation of bioactive mixtures with acetylcholine binding protein (AChBP) affinity analysis with help of a spotter technology. The high-resolution fractionation tailors the fractionation frequency to the chromatographic peaks. Postcolumn reagents for AChBP bioaffinity profiling are mixed prior to droplet ejection into 1536-well plates. after an incubation step, microplate reader analysis is used to determine bioactive compounds in a mixture. For ligands tested, a good correlation was found for IC
Original languageEnglish
Pages (from-to)917-924
JournalJournal of Biomolecular Screening
Volume16
DOIs
Publication statusPublished - 2011

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