High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.

J. Kool; F. Heus; G.E. de Kloe; H. Lingeman; A.B. Smit; R. Leurs; I.J.P. de Esch; H. Irth; W.M.A. Niessen

doi:10.1177/1087057111413921

High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.

J. Kool, F. Heus, G.E. de Kloe, H. Lingeman, A.B. Smit, R. Leurs, I.J.P. de Esch, H. Irth, W.M.A. Niessen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

This study describes the evaluation, validation, and use of contactless postcolumn fractionation of bioactive mixtures with acetylcholine binding protein (AChBP) affinity analysis with help of a spotter technology. The high-resolution fractionation tailors the fractionation frequency to the chromatographic peaks. Postcolumn reagents for AChBP bioaffinity profiling are mixed prior to droplet ejection into 1536-well plates. after an incubation step, microplate reader analysis is used to determine bioactive compounds in a mixture. For ligands tested, a good correlation was found for IC

Original language	English
Pages (from-to)	917-924
Journal	Journal of Biomolecular Screening
Volume	16
DOIs	https://doi.org/10.1177/1087057111413921
Publication status	Published - 2011

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10.1177/1087057111413921

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title = "High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.",

abstract = "This study describes the evaluation, validation, and use of contactless postcolumn fractionation of bioactive mixtures with acetylcholine binding protein (AChBP) affinity analysis with help of a spotter technology. The high-resolution fractionation tailors the fractionation frequency to the chromatographic peaks. Postcolumn reagents for AChBP bioaffinity profiling are mixed prior to droplet ejection into 1536-well plates. after an incubation step, microplate reader analysis is used to determine bioactive compounds in a mixture. For ligands tested, a good correlation was found for IC",

author = "J. Kool and F. Heus and {de Kloe}, G.E. and H. Lingeman and A.B. Smit and R. Leurs and {de Esch}, I.J.P. and H. Irth and W.M.A. Niessen",

year = "2011",

doi = "10.1177/1087057111413921",

language = "English",

volume = "16",

pages = "917--924",

journal = "Journal of Biomolecular Screening",

issn = "1087-0571",

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TY - JOUR

T1 - High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.

AU - Kool, J.

AU - Heus, F.

AU - de Kloe, G.E.

AU - Lingeman, H.

AU - Smit, A.B.

AU - Leurs, R.

AU - de Esch, I.J.P.

AU - Irth, H.

AU - Niessen, W.M.A.

PY - 2011

Y1 - 2011

N2 - This study describes the evaluation, validation, and use of contactless postcolumn fractionation of bioactive mixtures with acetylcholine binding protein (AChBP) affinity analysis with help of a spotter technology. The high-resolution fractionation tailors the fractionation frequency to the chromatographic peaks. Postcolumn reagents for AChBP bioaffinity profiling are mixed prior to droplet ejection into 1536-well plates. after an incubation step, microplate reader analysis is used to determine bioactive compounds in a mixture. For ligands tested, a good correlation was found for IC

AB - This study describes the evaluation, validation, and use of contactless postcolumn fractionation of bioactive mixtures with acetylcholine binding protein (AChBP) affinity analysis with help of a spotter technology. The high-resolution fractionation tailors the fractionation frequency to the chromatographic peaks. Postcolumn reagents for AChBP bioaffinity profiling are mixed prior to droplet ejection into 1536-well plates. after an incubation step, microplate reader analysis is used to determine bioactive compounds in a mixture. For ligands tested, a good correlation was found for IC

U2 - 10.1177/1087057111413921

DO - 10.1177/1087057111413921

M3 - Article

SN - 1087-0571

VL - 16

SP - 917

EP - 924

JO - Journal of Biomolecular Screening

JF - Journal of Biomolecular Screening

ER -

High-resolution bioactivity profiling of mixtures towards the acetylcholine binding protein aided by nanofractionation spotter technology.

Abstract

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