High-resolution metabolic profiling towards G-protein coupled receptors: rapid and comprehensive screening of histamine H4 receptor ligands

J. Kool, A.F. Rudebeck, F Fleurbaaij, S. Nijmeijer, D. Falck, R.A. Smits, H.F. Vischer, R. Leurs, W.M.A. Niessen

Research output: Contribution to JournalArticleAcademicpeer-review


In the past years we developed high-resolution screening platforms involving separation of bioactive mixtures and on-line or at-line bioassays for a wide variety of biological targets with parallel mass spectrometric detection and identification. In the current research, we make a major step forward in the development of at-line bioassays by implementation of radioligand receptor binding and functional cellular assays to evaluate bioactvity and selectivity. We demonstrate a new platform for high-resolution metabolic profiling of lead compounds for functional activity and selectivity toward the human histamine H
Original languageEnglish
Pages (from-to)213-220
JournalJournal of Chromatography A
Early online date22 Mar 2012
Publication statusPublished - 2012


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