High-resolution real-time dual-view imaging with multiple point of view microscopy

P.J.J. Mangeol, E.J.G. Peterman

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.
Original languageEnglish
Pages (from-to)3631-3642
JournalBiomedical Optics Express
Volume7
Issue number9
DOIs
Publication statusPublished - 2016

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