Abstract
Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.
Original language | English |
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Pages (from-to) | 3631-3642 |
Journal | Biomedical Optics Express |
Volume | 7 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2016 |