High-resolution real-time dual-view imaging with multiple point of view microscopy

P.J.J. Mangeol; E.J.G. Peterman

doi:10.1364/BOE.7.003631

High-resolution real-time dual-view imaging with multiple point of view microscopy

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.

Original language	English
Pages (from-to)	3631-3642
Journal	Biomedical Optics Express
Volume	7
Issue number	9
DOIs	https://doi.org/10.1364/BOE.7.003631
Publication status	Published - 2016

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title = "High-resolution real-time dual-view imaging with multiple point of view microscopy",

abstract = "Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.",

author = "P.J.J. Mangeol and E.J.G. Peterman",

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AU - Peterman, E.J.G.

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AB - Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.

U2 - 10.1364/BOE.7.003631

DO - 10.1364/BOE.7.003631

M3 - Article

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EP - 3642

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 9

ER -

High-resolution real-time dual-view imaging with multiple point of view microscopy

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