High-speed multi-wavelength Fresnel diffraction imaging

D.W.E. Noom, D.E. Boonzajer Flaes, E. Labordus, K.S.E. Eikema, S.M. Witte

Research output: Contribution to JournalArticleAcademicpeer-review


We demonstrate a compact lensless microscope which can capture video-rate phase contrast images of moving objects and allows numerical scanning of the focal distance after recording. Using only an RGB-detector and illumination from a single mode fiber, diffraction patterns at three wavelengths are recorded simultaneously, enabling high-speed data collection and reconstruction of phase and amplitude. The technique is used for imaging of a moving test target, beads in a flow cell, and imaging of Caenorhabditis elegans moving in a droplet of liquid.
Original languageEnglish
Pages (from-to)30504-30511
JournalOptics Express
Issue number25
Publication statusPublished - 2014


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