Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

L. Cheng, X. Lei, Z. Yang, Y. Kong, P. Xu, S. Peng, J. Wang, C. Chen, Y. Dong, X. Hu, X. Zhang, T. Forouzanfar, G. Wu, X. Fu

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

© 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.Objectives: Histatin 1(Hst 1) has been proved to promote wound healing. However, there was no specific study on the regulation made by Hst 1 of fibroblasts in the process of wound healing. This research comprehensively studied the regulation of Hst 1 on the function of fibroblasts in the process of wound healing and preliminary mechanism about it. Materials and methods: The full-thickness skin wound model was made on the back of C57/BL6 mice. The wound healing, collagen deposition and fibroblast distribution were detected on days 3, 5 and 7 after injury. Fibroblast was cultured in vitro and stimulated with Hst 1, and then, their biological characteristics and functions were detected. Results: Histatin 1 can effectively promote wound healing, improve collagen deposition during and after healing and increase the number and function of fibroblasts. After healing, the mechanical properties of the skin also improved. In vitro, the migration ability of fibroblasts stimulated by Hst 1 was significantly improved, and the fibroblasts transformed more into myofibroblasts, which improved the function of contraction and collagen secretion. In fibroblasts, mTOR signalling pathway can be activated by Hst 1. Conclusions: Histatin 1 can accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. The intermolecular mechanisms need to be further studied, and this study provides a direction about mTOR signalling pathway.
Original languageEnglish
Article numbere13087
JournalCell Proliferation
Volume54
Issue number8
DOIs
Publication statusPublished - 1 Aug 2021

Funding

This research was funded by the National Nature Science Foundation of China (81830064, 81721092), the National Key Research and Development Plan (2017YFC1103300), the CAMS Innovation Fund for Medical Sciences (CIFMS, 2019‐I2M‐5‐059) and the Military Medical Research and Development Projects (AWS17J005, 2019‐126), Key Research and Development Plan of Zhejiang Province (Grant No. 2021C04013) The National Nature Science Foundation of China (81830064, 81721092), the National Key Research and Development Plan (2017YFC1103300), the CAMS Innovation Fund for Medical Sciences (CIFMS, 2019‐I2M‐5‐059) and the Military Medical Research and Development Projects (AWS17J005, 2019‐126), Key Research and Development Plan of Zhejiang Province (Grant No. 2021C04013)

FundersFunder number
Military Medical Research and Development Projects2019‐126, AWS17J005
Key Technology Research and Development Program of Shandong2021C04013
National Natural Science Foundation of China81830064, 81721092
Chinese Academy of Meteorological Sciences2019‐I2M‐5‐059
National Key Research and Development Program of China2017YFC1103300

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