Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

L. Cheng; X. Lei; Z. Yang; Y. Kong; P. Xu; S. Peng; J. Wang; C. Chen; Y. Dong; X. Hu; X. Zhang; T. Forouzanfar; G. Wu; X. Fu

doi:10.1111/cpr.13087

Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

L. Cheng, X. Lei, Z. Yang, Y. Kong, P. Xu, S. Peng, J. Wang, C. Chen, Y. Dong, X. Hu, X. Zhang, T. Forouzanfar, G. Wu, X. Fu

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Objectives: Histatin 1(Hst 1) has been proved to promote wound healing. However, there was no specific study on the regulation made by Hst 1 of fibroblasts in the process of wound healing. This research comprehensively studied the regulation of Hst 1 on the function of fibroblasts in the process of wound healing and preliminary mechanism about it.

Materials and methods: The full-thickness skin wound model was made on the back of C57/BL6 mice. The wound healing, collagen deposition and fibroblast distribution were detected on days 3, 5 and 7 after injury. Fibroblast was cultured in vitro and stimulated with Hst 1, and then, their biological characteristics and functions were detected.

Results: Histatin 1 can effectively promote wound healing, improve collagen deposition during and after healing and increase the number and function of fibroblasts. After healing, the mechanical properties of the skin also improved. In vitro, the migration ability of fibroblasts stimulated by Hst 1 was significantly improved, and the fibroblasts transformed more into myofibroblasts, which improved the function of contraction and collagen secretion. In fibroblasts, mTOR signalling pathway can be activated by Hst 1.

Conclusions: Histatin 1 can accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. The intermolecular mechanisms need to be further studied, and this study provides a direction about mTOR signalling pathway.

Original language	English
Article number	e13087
Pages (from-to)	1-12
Number of pages	12
Journal	Cell Proliferation
Volume	54
Issue number	8
Early online date	13 Jul 2021
DOIs	https://doi.org/10.1111/cpr.13087
Publication status	Published - Aug 2021

Bibliographical note

Funding

This research was funded by the National Nature Science Foundation of China (81830064, 81721092), the National Key Research and Development Plan (2017YFC1103300), the CAMS Innovation Fund for Medical Sciences (CIFMS, 2019‐I2M‐5‐059) and the Military Medical Research and Development Projects (AWS17J005, 2019‐126), Key Research and Development Plan of Zhejiang Province (Grant No. 2021C04013) The National Nature Science Foundation of China (81830064, 81721092), the National Key Research and Development Plan (2017YFC1103300), the CAMS Innovation Fund for Medical Sciences (CIFMS, 2019‐I2M‐5‐059) and the Military Medical Research and Development Projects (AWS17J005, 2019‐126), Key Research and Development Plan of Zhejiang Province (Grant No. 2021C04013)

Funders	Funder number
Military Medical Research and Development Projects	2019‐126, AWS17J005
Key Technology Research and Development Program of Shandong	2021C04013
National Natural Science Foundation of China	81830064, 81721092
Chinese Academy of Meteorological Sciences	2019‐I2M‐5‐059
National Key Research and Development Program of China	2017YFC1103300

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title = "Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast",

abstract = "Objectives: Histatin 1(Hst 1) has been proved to promote wound healing. However, there was no specific study on the regulation made by Hst 1 of fibroblasts in the process of wound healing. This research comprehensively studied the regulation of Hst 1 on the function of fibroblasts in the process of wound healing and preliminary mechanism about it. Materials and methods: The full-thickness skin wound model was made on the back of C57/BL6 mice. The wound healing, collagen deposition and fibroblast distribution were detected on days 3, 5 and 7 after injury. Fibroblast was cultured in vitro and stimulated with Hst 1, and then, their biological characteristics and functions were detected. Results: Histatin 1 can effectively promote wound healing, improve collagen deposition during and after healing and increase the number and function of fibroblasts. After healing, the mechanical properties of the skin also improved. In vitro, the migration ability of fibroblasts stimulated by Hst 1 was significantly improved, and the fibroblasts transformed more into myofibroblasts, which improved the function of contraction and collagen secretion. In fibroblasts, mTOR signalling pathway can be activated by Hst 1. Conclusions: Histatin 1 can accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. The intermolecular mechanisms need to be further studied, and this study provides a direction about mTOR signalling pathway.",

author = "L. Cheng and X. Lei and Z. Yang and Y. Kong and P. Xu and S. Peng and J. Wang and C. Chen and Y. Dong and X. Hu and X. Zhang and T. Forouzanfar and G. Wu and X. Fu",

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AU - Cheng, L.

AU - Lei, X.

AU - Yang, Z.

AU - Kong, Y.

AU - Xu, P.

AU - Peng, S.

AU - Wang, J.

AU - Chen, C.

AU - Dong, Y.

AU - Hu, X.

AU - Zhang, X.

AU - Forouzanfar, T.

AU - Wu, G.

AU - Fu, X.

PY - 2021/8

Y1 - 2021/8

N2 - Objectives: Histatin 1(Hst 1) has been proved to promote wound healing. However, there was no specific study on the regulation made by Hst 1 of fibroblasts in the process of wound healing. This research comprehensively studied the regulation of Hst 1 on the function of fibroblasts in the process of wound healing and preliminary mechanism about it. Materials and methods: The full-thickness skin wound model was made on the back of C57/BL6 mice. The wound healing, collagen deposition and fibroblast distribution were detected on days 3, 5 and 7 after injury. Fibroblast was cultured in vitro and stimulated with Hst 1, and then, their biological characteristics and functions were detected. Results: Histatin 1 can effectively promote wound healing, improve collagen deposition during and after healing and increase the number and function of fibroblasts. After healing, the mechanical properties of the skin also improved. In vitro, the migration ability of fibroblasts stimulated by Hst 1 was significantly improved, and the fibroblasts transformed more into myofibroblasts, which improved the function of contraction and collagen secretion. In fibroblasts, mTOR signalling pathway can be activated by Hst 1. Conclusions: Histatin 1 can accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. The intermolecular mechanisms need to be further studied, and this study provides a direction about mTOR signalling pathway.

AB - Objectives: Histatin 1(Hst 1) has been proved to promote wound healing. However, there was no specific study on the regulation made by Hst 1 of fibroblasts in the process of wound healing. This research comprehensively studied the regulation of Hst 1 on the function of fibroblasts in the process of wound healing and preliminary mechanism about it. Materials and methods: The full-thickness skin wound model was made on the back of C57/BL6 mice. The wound healing, collagen deposition and fibroblast distribution were detected on days 3, 5 and 7 after injury. Fibroblast was cultured in vitro and stimulated with Hst 1, and then, their biological characteristics and functions were detected. Results: Histatin 1 can effectively promote wound healing, improve collagen deposition during and after healing and increase the number and function of fibroblasts. After healing, the mechanical properties of the skin also improved. In vitro, the migration ability of fibroblasts stimulated by Hst 1 was significantly improved, and the fibroblasts transformed more into myofibroblasts, which improved the function of contraction and collagen secretion. In fibroblasts, mTOR signalling pathway can be activated by Hst 1. Conclusions: Histatin 1 can accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. The intermolecular mechanisms need to be further studied, and this study provides a direction about mTOR signalling pathway.

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Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

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