Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease

A.E. Oostlander, N. Bravenboer, E. Sohl, P.J. Holzmann, C.J. van der Woude, G. Dijkstra, P.C.F. Stokkers, B. Oldenburg, J.C. Netelenbos, D.W. Hommes, A.A. van Bodegraven, P.T.A.M. Lips

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Background & Aims: Crohn's disease (CD) is associated with an increased prevalence of osteoporosis, but the pathogenesis of this bone loss is only partly understood. We assessed bone structure and remodeling at the tissue level in patients with quiescent CD. We also investigated the roles of osteocyte density and apoptosis in CD-associated bone loss. Methods: The study included 23 patients with quiescent CD; this was a subgroup of patients from a large randomized, double-blind, placebo-controlled, multicenter trial. We obtained transiliac bone biopsy samples and performed histomorphometric analysis. Results: were compared with data from age- and sex-matched healthy individuals (controls). Results: Trabecular bone volume was decreased among patients with CD compared with controls (18.90% vs 25.49%; P < .001). The low bone volume was characterized by decreased trabecular thickness (120.61 vs 151.42 μm; P < .01). Bone formation and resorption were reduced, as indicated by a decreased mineral apposition rate (0.671 vs 0.746 μm/day; P < .01) and a low osteoclast number and surface area compared with controls and published values, respectively. In trabecular bone of patients with CD, osteocyte density and apoptosis were normal. The percentage of empty lacunae among patients was higher than that of published values in controls. Conclusions:: In adult patients with quiescent CD, bone histomorphometric analysis revealed a reduction in bone mass that was characterized by trabecular thinning. The CD-associated bone loss was caused by reduced bone formation, possibly as a consequence of decreased osteocyte viability in the patients' past. © 2011 AGA Institute.
    Original languageEnglish
    Pages (from-to)116-123
    JournalGastroenterology
    Volume140
    Issue number1
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Osteogenesis
    Crohn Disease
    Bone and Bones
    Osteocytes
    Apoptosis
    Bone Remodeling
    Osteoclasts
    Bone Resorption
    Osteoporosis
    Multicenter Studies
    Minerals
    Placebos
    Biopsy

    Cite this

    Oostlander, A. E., Bravenboer, N., Sohl, E., Holzmann, P. J., van der Woude, C. J., Dijkstra, G., ... Lips, P. T. A. M. (2011). Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease. Gastroenterology, 140(1), 116-123. https://doi.org/10.1053/j.gastro.2010.09.007
    Oostlander, A.E. ; Bravenboer, N. ; Sohl, E. ; Holzmann, P.J. ; van der Woude, C.J. ; Dijkstra, G. ; Stokkers, P.C.F. ; Oldenburg, B. ; Netelenbos, J.C. ; Hommes, D.W. ; van Bodegraven, A.A. ; Lips, P.T.A.M. / Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease. In: Gastroenterology. 2011 ; Vol. 140, No. 1. pp. 116-123.
    @article{cd42fe5c78f54b5ca4e441c0a44fef85,
    title = "Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease",
    abstract = "Background & Aims: Crohn's disease (CD) is associated with an increased prevalence of osteoporosis, but the pathogenesis of this bone loss is only partly understood. We assessed bone structure and remodeling at the tissue level in patients with quiescent CD. We also investigated the roles of osteocyte density and apoptosis in CD-associated bone loss. Methods: The study included 23 patients with quiescent CD; this was a subgroup of patients from a large randomized, double-blind, placebo-controlled, multicenter trial. We obtained transiliac bone biopsy samples and performed histomorphometric analysis. Results: were compared with data from age- and sex-matched healthy individuals (controls). Results: Trabecular bone volume was decreased among patients with CD compared with controls (18.90{\%} vs 25.49{\%}; P < .001). The low bone volume was characterized by decreased trabecular thickness (120.61 vs 151.42 μm; P < .01). Bone formation and resorption were reduced, as indicated by a decreased mineral apposition rate (0.671 vs 0.746 μm/day; P < .01) and a low osteoclast number and surface area compared with controls and published values, respectively. In trabecular bone of patients with CD, osteocyte density and apoptosis were normal. The percentage of empty lacunae among patients was higher than that of published values in controls. Conclusions:: In adult patients with quiescent CD, bone histomorphometric analysis revealed a reduction in bone mass that was characterized by trabecular thinning. The CD-associated bone loss was caused by reduced bone formation, possibly as a consequence of decreased osteocyte viability in the patients' past. {\circledC} 2011 AGA Institute.",
    author = "A.E. Oostlander and N. Bravenboer and E. Sohl and P.J. Holzmann and {van der Woude}, C.J. and G. Dijkstra and P.C.F. Stokkers and B. Oldenburg and J.C. Netelenbos and D.W. Hommes and {van Bodegraven}, A.A. and P.T.A.M. Lips",
    year = "2011",
    doi = "10.1053/j.gastro.2010.09.007",
    language = "English",
    volume = "140",
    pages = "116--123",
    journal = "Gastroenterology",
    issn = "0016-5085",
    publisher = "W.B. Saunders Ltd",
    number = "1",

    }

    Oostlander, AE, Bravenboer, N, Sohl, E, Holzmann, PJ, van der Woude, CJ, Dijkstra, G, Stokkers, PCF, Oldenburg, B, Netelenbos, JC, Hommes, DW, van Bodegraven, AA & Lips, PTAM 2011, 'Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease' Gastroenterology, vol. 140, no. 1, pp. 116-123. https://doi.org/10.1053/j.gastro.2010.09.007

    Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease. / Oostlander, A.E.; Bravenboer, N.; Sohl, E.; Holzmann, P.J.; van der Woude, C.J.; Dijkstra, G.; Stokkers, P.C.F.; Oldenburg, B.; Netelenbos, J.C.; Hommes, D.W.; van Bodegraven, A.A.; Lips, P.T.A.M.

    In: Gastroenterology, Vol. 140, No. 1, 2011, p. 116-123.

    Research output: Contribution to JournalArticleAcademicpeer-review

    TY - JOUR

    T1 - Histomorphometric Analysis Reveals Reduced Bone Mass and Bone Formation in Patients With Quiescent Crohn's Disease

    AU - Oostlander, A.E.

    AU - Bravenboer, N.

    AU - Sohl, E.

    AU - Holzmann, P.J.

    AU - van der Woude, C.J.

    AU - Dijkstra, G.

    AU - Stokkers, P.C.F.

    AU - Oldenburg, B.

    AU - Netelenbos, J.C.

    AU - Hommes, D.W.

    AU - van Bodegraven, A.A.

    AU - Lips, P.T.A.M.

    PY - 2011

    Y1 - 2011

    N2 - Background & Aims: Crohn's disease (CD) is associated with an increased prevalence of osteoporosis, but the pathogenesis of this bone loss is only partly understood. We assessed bone structure and remodeling at the tissue level in patients with quiescent CD. We also investigated the roles of osteocyte density and apoptosis in CD-associated bone loss. Methods: The study included 23 patients with quiescent CD; this was a subgroup of patients from a large randomized, double-blind, placebo-controlled, multicenter trial. We obtained transiliac bone biopsy samples and performed histomorphometric analysis. Results: were compared with data from age- and sex-matched healthy individuals (controls). Results: Trabecular bone volume was decreased among patients with CD compared with controls (18.90% vs 25.49%; P < .001). The low bone volume was characterized by decreased trabecular thickness (120.61 vs 151.42 μm; P < .01). Bone formation and resorption were reduced, as indicated by a decreased mineral apposition rate (0.671 vs 0.746 μm/day; P < .01) and a low osteoclast number and surface area compared with controls and published values, respectively. In trabecular bone of patients with CD, osteocyte density and apoptosis were normal. The percentage of empty lacunae among patients was higher than that of published values in controls. Conclusions:: In adult patients with quiescent CD, bone histomorphometric analysis revealed a reduction in bone mass that was characterized by trabecular thinning. The CD-associated bone loss was caused by reduced bone formation, possibly as a consequence of decreased osteocyte viability in the patients' past. © 2011 AGA Institute.

    AB - Background & Aims: Crohn's disease (CD) is associated with an increased prevalence of osteoporosis, but the pathogenesis of this bone loss is only partly understood. We assessed bone structure and remodeling at the tissue level in patients with quiescent CD. We also investigated the roles of osteocyte density and apoptosis in CD-associated bone loss. Methods: The study included 23 patients with quiescent CD; this was a subgroup of patients from a large randomized, double-blind, placebo-controlled, multicenter trial. We obtained transiliac bone biopsy samples and performed histomorphometric analysis. Results: were compared with data from age- and sex-matched healthy individuals (controls). Results: Trabecular bone volume was decreased among patients with CD compared with controls (18.90% vs 25.49%; P < .001). The low bone volume was characterized by decreased trabecular thickness (120.61 vs 151.42 μm; P < .01). Bone formation and resorption were reduced, as indicated by a decreased mineral apposition rate (0.671 vs 0.746 μm/day; P < .01) and a low osteoclast number and surface area compared with controls and published values, respectively. In trabecular bone of patients with CD, osteocyte density and apoptosis were normal. The percentage of empty lacunae among patients was higher than that of published values in controls. Conclusions:: In adult patients with quiescent CD, bone histomorphometric analysis revealed a reduction in bone mass that was characterized by trabecular thinning. The CD-associated bone loss was caused by reduced bone formation, possibly as a consequence of decreased osteocyte viability in the patients' past. © 2011 AGA Institute.

    U2 - 10.1053/j.gastro.2010.09.007

    DO - 10.1053/j.gastro.2010.09.007

    M3 - Article

    VL - 140

    SP - 116

    EP - 123

    JO - Gastroenterology

    JF - Gastroenterology

    SN - 0016-5085

    IS - 1

    ER -