Hst1/Gel-MA Scaffold Significantly Promotes the Quality of Osteochondral Regeneration in the Temporomandibular Joint

Yiyang Du; Menghan Chen; Jing Jiang; Lei Wang; Gang Wu; Jianying Feng

doi:10.3390/jfb14100513

Hst1/Gel-MA Scaffold Significantly Promotes the Quality of Osteochondral Regeneration in the Temporomandibular Joint

Yiyang Du
, Menghan Chen
, Jing Jiang
, Lei Wang
, Gang Wu^*
, Jianying Feng^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Objective: Our aim was to evaluate the capacity of the human salivary histatin-1-functionalized methacrylic gelatin scaffold to control osteochondral tissue regeneration and repair in vivo in rabbits with major temporomandibular joint dimensional abnormalities. Materials and Methods: In order to compare human salivary histatin-1-functionalized methacrylic gelatin scaffolds to the Blank and Gel-MA hydrogel groups, scaffolds were implanted into osteochondral lesions of a critical size (3 × 3 mm) in the anterior region of the condyle of the temporomandibular joint in New Zealand white rabbits. At 4 weeks after implantation, the repair was evaluated using macroscopic examination, histology, and micro-CT analysis. Results: In the comparison of the composite scaffold group with the Blank and Gel-MA groups, analysis of the healed tissue revealed an improved macroscopic appearance in the composite scaffold group. Regeneration was induced by host cell migration in the Hst1/Gel-MA scaffold group. Conclusions: The current study offers a viable method for in vivo cartilage repair that does not require cell transplantation. Future clinical applications of this strategy’s optimization have many potential advantages.

Original language	English
Article number	513
Pages (from-to)	1-15
Number of pages	15
Journal	Journal of Functional Biomaterials
Volume	14
Issue number	10
Early online date	12 Oct 2023
DOIs	https://doi.org/10.3390/jfb14100513
Publication status	Published - Oct 2023

Bibliographical note

Funding Information:
This research was funded by the Basic Public Welfare Research Project of Zhejiang Province (No. LGF22H140007); Wenzhou Science and Technology Foundation (No. Y20210266); and the Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province (No. WIUCASK20004).

Publisher Copyright:
© 2023 by the authors.

Funding

This research was funded by the Basic Public Welfare Research Project of Zhejiang Province (No. LGF22H140007); Wenzhou Science and Technology Foundation (No. Y20210266); and the Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province (No. WIUCASK20004).

Funders	Funder number
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province	WIUCASK20004
Wenzhou Science and Technology Foundation	Y20210266
Basic Public Welfare Research Program of Zhejiang Province	LGF22H140007
Basic Public Welfare Research Program of Zhejiang Province

Keywords

cartilage
in vivo
osteochondral
tissue engineering

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10.3390/jfb14100513

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title = "Hst1/Gel-MA Scaffold Significantly Promotes the Quality of Osteochondral Regeneration in the Temporomandibular Joint",

abstract = "Objective: Our aim was to evaluate the capacity of the human salivary histatin-1-functionalized methacrylic gelatin scaffold to control osteochondral tissue regeneration and repair in vivo in rabbits with major temporomandibular joint dimensional abnormalities. Materials and Methods: In order to compare human salivary histatin-1-functionalized methacrylic gelatin scaffolds to the Blank and Gel-MA hydrogel groups, scaffolds were implanted into osteochondral lesions of a critical size (3 × 3 mm) in the anterior region of the condyle of the temporomandibular joint in New Zealand white rabbits. At 4 weeks after implantation, the repair was evaluated using macroscopic examination, histology, and micro-CT analysis. Results: In the comparison of the composite scaffold group with the Blank and Gel-MA groups, analysis of the healed tissue revealed an improved macroscopic appearance in the composite scaffold group. Regeneration was induced by host cell migration in the Hst1/Gel-MA scaffold group. Conclusions: The current study offers a viable method for in vivo cartilage repair that does not require cell transplantation. Future clinical applications of this strategy{\textquoteright}s optimization have many potential advantages.",

keywords = "cartilage, in vivo, osteochondral, tissue engineering",

author = "Yiyang Du and Menghan Chen and Jing Jiang and Lei Wang and Gang Wu and Jianying Feng",

note = "Funding Information: This research was funded by the Basic Public Welfare Research Project of Zhejiang Province (No. LGF22H140007); Wenzhou Science and Technology Foundation (No. Y20210266); and the Engineering Research Center of Clinical Functional Materials and Diagnosis \& Treatment Devices of Zhejiang Province (No. WIUCASK20004). Publisher Copyright: {\textcopyright} 2023 by the authors.",

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doi = "10.3390/jfb14100513",

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T1 - Hst1/Gel-MA Scaffold Significantly Promotes the Quality of Osteochondral Regeneration in the Temporomandibular Joint

AU - Du, Yiyang

AU - Chen, Menghan

AU - Jiang, Jing

AU - Wang, Lei

AU - Wu, Gang

AU - Feng, Jianying

N1 - Funding Information: This research was funded by the Basic Public Welfare Research Project of Zhejiang Province (No. LGF22H140007); Wenzhou Science and Technology Foundation (No. Y20210266); and the Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province (No. WIUCASK20004). Publisher Copyright: © 2023 by the authors.

PY - 2023/10

Y1 - 2023/10

N2 - Objective: Our aim was to evaluate the capacity of the human salivary histatin-1-functionalized methacrylic gelatin scaffold to control osteochondral tissue regeneration and repair in vivo in rabbits with major temporomandibular joint dimensional abnormalities. Materials and Methods: In order to compare human salivary histatin-1-functionalized methacrylic gelatin scaffolds to the Blank and Gel-MA hydrogel groups, scaffolds were implanted into osteochondral lesions of a critical size (3 × 3 mm) in the anterior region of the condyle of the temporomandibular joint in New Zealand white rabbits. At 4 weeks after implantation, the repair was evaluated using macroscopic examination, histology, and micro-CT analysis. Results: In the comparison of the composite scaffold group with the Blank and Gel-MA groups, analysis of the healed tissue revealed an improved macroscopic appearance in the composite scaffold group. Regeneration was induced by host cell migration in the Hst1/Gel-MA scaffold group. Conclusions: The current study offers a viable method for in vivo cartilage repair that does not require cell transplantation. Future clinical applications of this strategy’s optimization have many potential advantages.

AB - Objective: Our aim was to evaluate the capacity of the human salivary histatin-1-functionalized methacrylic gelatin scaffold to control osteochondral tissue regeneration and repair in vivo in rabbits with major temporomandibular joint dimensional abnormalities. Materials and Methods: In order to compare human salivary histatin-1-functionalized methacrylic gelatin scaffolds to the Blank and Gel-MA hydrogel groups, scaffolds were implanted into osteochondral lesions of a critical size (3 × 3 mm) in the anterior region of the condyle of the temporomandibular joint in New Zealand white rabbits. At 4 weeks after implantation, the repair was evaluated using macroscopic examination, histology, and micro-CT analysis. Results: In the comparison of the composite scaffold group with the Blank and Gel-MA groups, analysis of the healed tissue revealed an improved macroscopic appearance in the composite scaffold group. Regeneration was induced by host cell migration in the Hst1/Gel-MA scaffold group. Conclusions: The current study offers a viable method for in vivo cartilage repair that does not require cell transplantation. Future clinical applications of this strategy’s optimization have many potential advantages.

KW - cartilage

KW - in vivo

KW - osteochondral

KW - tissue engineering

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Hst1/Gel-MA Scaffold Significantly Promotes the Quality of Osteochondral Regeneration in the Temporomandibular Joint

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