Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

Wei Sun; Dandan Ma; Jan G.M. Bolscher; Kamran Nazmi; Enno C.I. Veerman; Floris J. Bikker; Ping Sun; Haiyan Lin; Gang Wu

doi:10.3389/fbioe.2020.584410

Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

Wei Sun, Dandan Ma, Jan G.M. Bolscher, Kamran Nazmi, Enno C.I. Veerman, Floris J. Bikker, Ping Sun, Haiyan Lin^*, Gang Wu

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways involved. Osteogenic cells were seeded on bio-inert glass slides with or without the presence of Hst1 in dose-dependent or time-course assays. 1 scrambled and 6 truncated Hst1 variants were also evaluated. Cell spreading was analyzed using a well-established point-counting method. Fluorescent microscopy was adopted to examine the cellular uptake of fluorescently labeled Hst1 (F-Hst1) and also the cell spreading on sandblasted and acid etched titanium surfaces. Signaling inhibitors, such as U0126, SB203580, and pertussis toxin (PTx) were used to identify the potential role of extracellular-signal-regulated kinase, p38 and G protein-coupled receptor pathways, respectively. After 60 min incubation, Hst1 significantly promoted the spreading of osteogenic cells with an optimal concentration of 10 μM, while truncated and scrambled Hst1 did not. F-Hst1 was taken up and localized in the vicinity of the nuclei. U0126 and SB2030580, but not PTx, inhibited the effect of Hst1. 10 μM Hst1 significantly promoted the spreading of osteogenic cells on both bio-inert substrates and titanium SLA surfaces, which involved ERK and p38 signaling. Human salivary histatin-1 might be a promising peptide to enhance bone healing and implant osteointegration in clinic.

Original language	English
Article number	584410
Pages (from-to)	1-10
Number of pages	10
Journal	Frontiers in Bioengineering and Biotechnology
Volume	8
Issue number	October
Early online date	23 Oct 2020
DOIs	https://doi.org/10.3389/fbioe.2020.584410
Publication status	Published - Oct 2020

Bibliographical note

Funding Information:
This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China’s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104).

Funding Information:
Funding. This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China?s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104).

Publisher Copyright:
© Copyright © 2020 Sun, Ma, Bolscher, Nazmi, Veerman, Bikker, Sun, Lin and Wu.

Funding

This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China’s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104). Funding. This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China?s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104).

Keywords

cell spreading
osteoconductivity
osteogenic cells
peptide
salivary

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3389/fbioe.2020.584410

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title = "Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces",

abstract = "Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways involved. Osteogenic cells were seeded on bio-inert glass slides with or without the presence of Hst1 in dose-dependent or time-course assays. 1 scrambled and 6 truncated Hst1 variants were also evaluated. Cell spreading was analyzed using a well-established point-counting method. Fluorescent microscopy was adopted to examine the cellular uptake of fluorescently labeled Hst1 (F-Hst1) and also the cell spreading on sandblasted and acid etched titanium surfaces. Signaling inhibitors, such as U0126, SB203580, and pertussis toxin (PTx) were used to identify the potential role of extracellular-signal-regulated kinase, p38 and G protein-coupled receptor pathways, respectively. After 60 min incubation, Hst1 significantly promoted the spreading of osteogenic cells with an optimal concentration of 10 μM, while truncated and scrambled Hst1 did not. F-Hst1 was taken up and localized in the vicinity of the nuclei. U0126 and SB2030580, but not PTx, inhibited the effect of Hst1. 10 μM Hst1 significantly promoted the spreading of osteogenic cells on both bio-inert substrates and titanium SLA surfaces, which involved ERK and p38 signaling. Human salivary histatin-1 might be a promising peptide to enhance bone healing and implant osteointegration in clinic.",

keywords = "cell spreading, osteoconductivity, osteogenic cells, peptide, salivary",

author = "Wei Sun and Dandan Ma and Bolscher, {Jan G.M.} and Kamran Nazmi and Veerman, {Enno C.I.} and Bikker, {Floris J.} and Ping Sun and Haiyan Lin and Gang Wu",

note = "Funding Information: This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China{\textquoteright}s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104). Funding Information: Funding. This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China?s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Sun, Ma, Bolscher, Nazmi, Veerman, Bikker, Sun, Lin and Wu.",

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AU - Nazmi, Kamran

AU - Veerman, Enno C.I.

AU - Bikker, Floris J.

AU - Sun, Ping

AU - Lin, Haiyan

AU - Wu, Gang

N1 - Funding Information: This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China’s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104). Funding Information: Funding. This work was supported by the Zhejiang Provincial Natural Science Foundation of China (grant no. LQ18H140003), the Foundation of Zhejiang Educational Committee, China (grant no. Y201636248), Zhejiang Provincial Natural Science Foundation of China (grant no. LQ Y17H140023), Zhejiang Provincial Basic Public Welfare Research Project (grant no. GJ19H140001), Science Technology Department of Zhejiang Province (grant no. 2017C33168). China?s National Key R&D Programs (NKPs, grant no. 2018YFB0407204), and Eurostars project (grant no. STAR19104). Publisher Copyright: © Copyright © 2020 Sun, Ma, Bolscher, Nazmi, Veerman, Bikker, Sun, Lin and Wu.

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N2 - Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways involved. Osteogenic cells were seeded on bio-inert glass slides with or without the presence of Hst1 in dose-dependent or time-course assays. 1 scrambled and 6 truncated Hst1 variants were also evaluated. Cell spreading was analyzed using a well-established point-counting method. Fluorescent microscopy was adopted to examine the cellular uptake of fluorescently labeled Hst1 (F-Hst1) and also the cell spreading on sandblasted and acid etched titanium surfaces. Signaling inhibitors, such as U0126, SB203580, and pertussis toxin (PTx) were used to identify the potential role of extracellular-signal-regulated kinase, p38 and G protein-coupled receptor pathways, respectively. After 60 min incubation, Hst1 significantly promoted the spreading of osteogenic cells with an optimal concentration of 10 μM, while truncated and scrambled Hst1 did not. F-Hst1 was taken up and localized in the vicinity of the nuclei. U0126 and SB2030580, but not PTx, inhibited the effect of Hst1. 10 μM Hst1 significantly promoted the spreading of osteogenic cells on both bio-inert substrates and titanium SLA surfaces, which involved ERK and p38 signaling. Human salivary histatin-1 might be a promising peptide to enhance bone healing and implant osteointegration in clinic.

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Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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Persistent URL (handle)

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