Hypertrophy of mature xenopus muscle fibres in culture induced by synergy of albumin and insulin

R.T. Jaspers, B.J. van Beek-Harmsen, M.A. Blankenstein, G. Goldspink, P.A.J.B.M. Huijing, W.J. van der Laarse

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. Single muscle fibres were dissected from m. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 μm) for 8 to 22 days. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200-600 nmol/L), (2) 0.2% bovine albumin or (3) 0.2% bovine albumin in combination with insulin (120 nmol/L). In culture medium with insulin, 50% of the muscle fibres became in-excitable within 7-12 days, whereas the other 50% were stable. Caffeine contractures of in-excitable muscle fibres produced 80.4±2.4% of initial peak tetanic force, indicating impaired excitation-contraction (E-C) coupling in in-excitable fibres. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In contrast, muscle fibres cultured with both albumin and insulin showed an increase in tetanic force and fibre cross-sectional area of 19.6±2.8% and 32.5±4.9%, respectively, (means±SEM.; P=0.007) after 16.3±1.7 days, whereas the number of sarcomeres in series remained unchanged. We conclude that albumin prevents muscle fibre damage and preserves E-C coupling in culture. Furthermore, albumin is important in regulating muscle fibre adaptation by a synergistic action with growth factors like insulin. © 2008 The Author(s).
    Original languageEnglish
    Pages (from-to)161-170
    JournalPflügers Archiv European Journal of Physiology
    Volume457
    Issue number1
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Xenopus
    Hypertrophy
    Muscle
    Albumins
    Insulin
    Muscles
    Fibers
    Sarcomeres
    Excitation Contraction Coupling
    Serum-Free Culture Media
    Xenopus laevis
    Contracture
    Somatomedins
    Caffeine
    Transducers
    Culture Media

    Cite this

    Jaspers, R.T. ; van Beek-Harmsen, B.J. ; Blankenstein, M.A. ; Goldspink, G. ; Huijing, P.A.J.B.M. ; van der Laarse, W.J. / Hypertrophy of mature xenopus muscle fibres in culture induced by synergy of albumin and insulin. In: Pflügers Archiv European Journal of Physiology. 2008 ; Vol. 457, No. 1. pp. 161-170.
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    abstract = "The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. Single muscle fibres were dissected from m. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 μm) for 8 to 22 days. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200-600 nmol/L), (2) 0.2{\%} bovine albumin or (3) 0.2{\%} bovine albumin in combination with insulin (120 nmol/L). In culture medium with insulin, 50{\%} of the muscle fibres became in-excitable within 7-12 days, whereas the other 50{\%} were stable. Caffeine contractures of in-excitable muscle fibres produced 80.4±2.4{\%} of initial peak tetanic force, indicating impaired excitation-contraction (E-C) coupling in in-excitable fibres. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In contrast, muscle fibres cultured with both albumin and insulin showed an increase in tetanic force and fibre cross-sectional area of 19.6±2.8{\%} and 32.5±4.9{\%}, respectively, (means±SEM.; P=0.007) after 16.3±1.7 days, whereas the number of sarcomeres in series remained unchanged. We conclude that albumin prevents muscle fibre damage and preserves E-C coupling in culture. Furthermore, albumin is important in regulating muscle fibre adaptation by a synergistic action with growth factors like insulin. {\circledC} 2008 The Author(s).",
    author = "R.T. Jaspers and {van Beek-Harmsen}, B.J. and M.A. Blankenstein and G. Goldspink and P.A.J.B.M. Huijing and {van der Laarse}, W.J.",
    year = "2008",
    doi = "10.1007/s00424-008-0499-0",
    language = "English",
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    pages = "161--170",
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    Hypertrophy of mature xenopus muscle fibres in culture induced by synergy of albumin and insulin. / Jaspers, R.T.; van Beek-Harmsen, B.J.; Blankenstein, M.A.; Goldspink, G.; Huijing, P.A.J.B.M.; van der Laarse, W.J.

    In: Pflügers Archiv European Journal of Physiology, Vol. 457, No. 1, 2008, p. 161-170.

    Research output: Contribution to JournalArticleAcademicpeer-review

    TY - JOUR

    T1 - Hypertrophy of mature xenopus muscle fibres in culture induced by synergy of albumin and insulin

    AU - Jaspers, R.T.

    AU - van Beek-Harmsen, B.J.

    AU - Blankenstein, M.A.

    AU - Goldspink, G.

    AU - Huijing, P.A.J.B.M.

    AU - van der Laarse, W.J.

    PY - 2008

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    N2 - The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. Single muscle fibres were dissected from m. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 μm) for 8 to 22 days. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200-600 nmol/L), (2) 0.2% bovine albumin or (3) 0.2% bovine albumin in combination with insulin (120 nmol/L). In culture medium with insulin, 50% of the muscle fibres became in-excitable within 7-12 days, whereas the other 50% were stable. Caffeine contractures of in-excitable muscle fibres produced 80.4±2.4% of initial peak tetanic force, indicating impaired excitation-contraction (E-C) coupling in in-excitable fibres. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In contrast, muscle fibres cultured with both albumin and insulin showed an increase in tetanic force and fibre cross-sectional area of 19.6±2.8% and 32.5±4.9%, respectively, (means±SEM.; P=0.007) after 16.3±1.7 days, whereas the number of sarcomeres in series remained unchanged. We conclude that albumin prevents muscle fibre damage and preserves E-C coupling in culture. Furthermore, albumin is important in regulating muscle fibre adaptation by a synergistic action with growth factors like insulin. © 2008 The Author(s).

    AB - The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. Single muscle fibres were dissected from m. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 μm) for 8 to 22 days. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200-600 nmol/L), (2) 0.2% bovine albumin or (3) 0.2% bovine albumin in combination with insulin (120 nmol/L). In culture medium with insulin, 50% of the muscle fibres became in-excitable within 7-12 days, whereas the other 50% were stable. Caffeine contractures of in-excitable muscle fibres produced 80.4±2.4% of initial peak tetanic force, indicating impaired excitation-contraction (E-C) coupling in in-excitable fibres. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In contrast, muscle fibres cultured with both albumin and insulin showed an increase in tetanic force and fibre cross-sectional area of 19.6±2.8% and 32.5±4.9%, respectively, (means±SEM.; P=0.007) after 16.3±1.7 days, whereas the number of sarcomeres in series remained unchanged. We conclude that albumin prevents muscle fibre damage and preserves E-C coupling in culture. Furthermore, albumin is important in regulating muscle fibre adaptation by a synergistic action with growth factors like insulin. © 2008 The Author(s).

    U2 - 10.1007/s00424-008-0499-0

    DO - 10.1007/s00424-008-0499-0

    M3 - Article

    VL - 457

    SP - 161

    EP - 170

    JO - Pflügers Archiv European Journal of Physiology

    JF - Pflügers Archiv European Journal of Physiology

    SN - 0031-6768

    IS - 1

    ER -