TY - GEN
T1 - Iberian pyrite belt subsurface life (IPBSL), a drilling project of biohydrometallurgical interest
AU - Amils, Ricardo
AU - Fernández-Remolar, David
AU - Parro, Victor
AU - Rodríguez-Manfredi, José Antonio
AU - Timmis, Ken
AU - Oggerin, Mónica
AU - Sánchez-Román, Mónica
AU - López, Francisco J.
AU - Fernández-Rodríguez, José Pablo
AU - Puente-Sánchez, Fernando
AU - Gómez-Ortiz, David
AU - Briones, Carlos
AU - Gómez, Felipe
AU - Omoregie, Enoma
AU - García-Villadangos, Miriam
AU - Rodríguez, Nuria
AU - Sanz, José Luis
PY - 2013
Y1 - 2013
N2 - The geomicrobiological characterization of Río Tinto, an extreme acidic environment, has proven the importance of the iron cycle, not only in generating the extreme conditions of the habitat (low pH, high concentration of toxic heavy metals) but also in maintaining the high level of microbial diversity detected in the water column and the sediments. The extreme conditions detected in the Tinto basin are not the product of industrial contamination but the consequence of the presence of an underground bioreactor that obtains its energy from the massive sulfide minerals of the Iberian Pyrite Belt (IPB). To test this hypothesis, a drilling project (IPBSL) to intersect ground waters interacting with the mineral ore is under way, to provide evidence of subsurface microbial activities. A dedicated geophysical characterization of the area selected two drilling sites due to the possible existence of water with high ionic content. Two wells have been drilled in Peña de Hierro, BH11 and BH10, with depths of 340 and 620 meters respectively, with recovery of cores and generation of samples in anaerobic and sterile conditions. The geological analysis of the retrieved cores showed an important alteration of mineral structures associated with the presence of water, with production of expected products from the bacterial oxidation of pyrite. Ion chromatography of water soluble compounds from uncontaminated samples showed the existence of putative electron donors, electron acceptors, as well as variable concentration of metabolic organic acids, which suggest the presence of an active subsurface ecosystem associated to the high sulfidic mineral content of the IPB. Enrichment cultures from selected samples showed evidences of an active iron and sulfur cycle, together with unexpected methanogenic, methanotrophic and acetogenic activities. The geological, geomicrobiological and molecular biology analyses which are under way, should allow the characterization of this ecosystem of biohydrometallurgical interest.
AB - The geomicrobiological characterization of Río Tinto, an extreme acidic environment, has proven the importance of the iron cycle, not only in generating the extreme conditions of the habitat (low pH, high concentration of toxic heavy metals) but also in maintaining the high level of microbial diversity detected in the water column and the sediments. The extreme conditions detected in the Tinto basin are not the product of industrial contamination but the consequence of the presence of an underground bioreactor that obtains its energy from the massive sulfide minerals of the Iberian Pyrite Belt (IPB). To test this hypothesis, a drilling project (IPBSL) to intersect ground waters interacting with the mineral ore is under way, to provide evidence of subsurface microbial activities. A dedicated geophysical characterization of the area selected two drilling sites due to the possible existence of water with high ionic content. Two wells have been drilled in Peña de Hierro, BH11 and BH10, with depths of 340 and 620 meters respectively, with recovery of cores and generation of samples in anaerobic and sterile conditions. The geological analysis of the retrieved cores showed an important alteration of mineral structures associated with the presence of water, with production of expected products from the bacterial oxidation of pyrite. Ion chromatography of water soluble compounds from uncontaminated samples showed the existence of putative electron donors, electron acceptors, as well as variable concentration of metabolic organic acids, which suggest the presence of an active subsurface ecosystem associated to the high sulfidic mineral content of the IPB. Enrichment cultures from selected samples showed evidences of an active iron and sulfur cycle, together with unexpected methanogenic, methanotrophic and acetogenic activities. The geological, geomicrobiological and molecular biology analyses which are under way, should allow the characterization of this ecosystem of biohydrometallurgical interest.
KW - Iberian pyrite belt
KW - Iron cycle
KW - Methanogenesis
KW - Subsurface geomicrobiology
KW - Sulfate reducing bacteria (SRB)
KW - Sulfur cycle
UR - http://www.scopus.com/inward/record.url?scp=84886780901&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886780901&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.825.15
DO - 10.4028/www.scientific.net/AMR.825.15
M3 - Conference contribution
AN - SCOPUS:84886780901
SN - 9783037858912
VL - 825
T3 - Advanced Materials Research
SP - 15
EP - 18
BT - Integration of Scientific and Industrial Knowledge on Biohydrometallurgy
T2 - 20th International Biohydrometallurgy Symposium, IBS 2013
Y2 - 8 October 2013 through 11 October 2013
ER -