Iberian pyrite belt subsurface life (IPBSL), a drilling project of biohydrometallurgical interest

Ricardo Amils*, David Fernández-Remolar, Victor Parro, José Antonio Rodríguez-Manfredi, Ken Timmis, Mónica Oggerin, Mónica Sánchez-Román, Francisco J. López, José Pablo Fernández-Rodríguez, Fernando Puente-Sánchez, David Gómez-Ortiz, Carlos Briones, Felipe Gómez, Enoma Omoregie, Miriam García-Villadangos, Nuria Rodríguez, José Luis Sanz

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingConference contributionAcademicpeer-review

Abstract

The geomicrobiological characterization of Río Tinto, an extreme acidic environment, has proven the importance of the iron cycle, not only in generating the extreme conditions of the habitat (low pH, high concentration of toxic heavy metals) but also in maintaining the high level of microbial diversity detected in the water column and the sediments. The extreme conditions detected in the Tinto basin are not the product of industrial contamination but the consequence of the presence of an underground bioreactor that obtains its energy from the massive sulfide minerals of the Iberian Pyrite Belt (IPB). To test this hypothesis, a drilling project (IPBSL) to intersect ground waters interacting with the mineral ore is under way, to provide evidence of subsurface microbial activities. A dedicated geophysical characterization of the area selected two drilling sites due to the possible existence of water with high ionic content. Two wells have been drilled in Peña de Hierro, BH11 and BH10, with depths of 340 and 620 meters respectively, with recovery of cores and generation of samples in anaerobic and sterile conditions. The geological analysis of the retrieved cores showed an important alteration of mineral structures associated with the presence of water, with production of expected products from the bacterial oxidation of pyrite. Ion chromatography of water soluble compounds from uncontaminated samples showed the existence of putative electron donors, electron acceptors, as well as variable concentration of metabolic organic acids, which suggest the presence of an active subsurface ecosystem associated to the high sulfidic mineral content of the IPB. Enrichment cultures from selected samples showed evidences of an active iron and sulfur cycle, together with unexpected methanogenic, methanotrophic and acetogenic activities. The geological, geomicrobiological and molecular biology analyses which are under way, should allow the characterization of this ecosystem of biohydrometallurgical interest.

Original languageEnglish
Title of host publicationIntegration of Scientific and Industrial Knowledge on Biohydrometallurgy
Pages15-18
Number of pages4
Volume825
DOIs
Publication statusPublished - 2013
Event20th International Biohydrometallurgy Symposium, IBS 2013 - Antofagasta, Chile
Duration: 8 Oct 201311 Oct 2013

Publication series

NameAdvanced Materials Research
Volume825
ISSN (Print)10226680

Conference

Conference20th International Biohydrometallurgy Symposium, IBS 2013
CountryChile
CityAntofagasta
Period8/10/1311/10/13

Keywords

  • Iberian pyrite belt
  • Iron cycle
  • Methanogenesis
  • Subsurface geomicrobiology
  • Sulfate reducing bacteria (SRB)
  • Sulfur cycle

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